Three-dimensional crystal structure of recombinant murine interferon-beta - PubMed (original) (raw)

Comparative Study

Three-dimensional crystal structure of recombinant murine interferon-beta

T Senda et al. EMBO J. 1992 Sep.

Abstract

The crystal structure of recombinant murine interferon-beta (IFN-beta) has been solved by the multiple isomorphous replacement method and refined to an R-factor of 20.5% against 2.6 A X-ray diffraction data. The structure shows a variant of the alpha-helix bundle with a new chain-folding topology, which seems to represent a basic structural framework of all the IFN-alpha and IFN-beta molecules belonging to the type I family. Functionally important segments of the polypeptide chain, as implied through numerous gene manipulation studies carried out so far, are spatially clustered indicating the binding site(s) to the receptor(s). Comparison of the present structure with those of other alpha-helical cytokine proteins, including porcine growth hormone, interleukin 2 and interferon gamma, indicated either a topological similarity in chain folding or a similar spatial arrangement of the alpha-helices.

PubMed Disclaimer

Similar articles

• Structural, functional and evolutionary implications of the three-dimensional crystal structure of murine interferon-beta.
  Mitsui Y, Senda T, Shimazu T, Matsuda S, Utsumi J. Mitsui Y, et al. Pharmacol Ther. 1993;58(1):93-132. doi: 10.1016/0163-7258(93)90068-o. Pharmacol Ther. 1993. PMID: 8415875 Review.
• Refined crystal structure of recombinant murine interferon-beta at 2.15 A resolution.
  Senda T, Saitoh S, Mitsui Y. Senda T, et al. J Mol Biol. 1995 Oct 13;253(1):187-207. doi: 10.1006/jmbi.1995.0544. J Mol Biol. 1995. PMID: 7473712
• Crystal structure of interleukin-10 reveals the functional dimer with an unexpected topological similarity to interferon gamma.
  Zdanov A, Schalk-Hihi C, Gustchina A, Tsang M, Weatherbee J, Wlodawer A. Zdanov A, et al. Structure. 1995 Jun 15;3(6):591-601. doi: 10.1016/s0969-2126(01)00193-9. Structure. 1995. PMID: 8590020
• Zinc mediated dimer of human interferon-alpha 2b revealed by X-ray crystallography.
  Radhakrishnan R, Walter LJ, Hruza A, Reichert P, Trotta PP, Nagabhushan TL, Walter MR. Radhakrishnan R, et al. Structure. 1996 Dec 15;4(12):1453-63. doi: 10.1016/s0969-2126(96)00152-9. Structure. 1996. PMID: 8994971
• Elucidation of the basic three-dimensional structure of type I interferons and its functional and evolutionary implications.
  Mitsui Y, Senda T. Mitsui Y, et al. J Interferon Cytokine Res. 1997 Jun;17(6):319-26. doi: 10.1089/jir.1997.17.319. J Interferon Cytokine Res. 1997. PMID: 9197998 Review.

Cited by

• The structure of granulocyte-colony-stimulating factor and its relationship to other growth factors.
  Hill CP, Osslund TD, Eisenberg D. Hill CP, et al. Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5167-71. doi: 10.1073/pnas.90.11.5167. Proc Natl Acad Sci U S A. 1993. PMID: 7685117 Free PMC article.
• Lambda Interferons: New Cytokines with Old Functions.
  Hamming OJ, Gad HH, Paludan S, Hartmann R. Hamming OJ, et al. Pharmaceuticals (Basel). 2010 Mar 25;3(4):795-809. doi: 10.3390/ph3040795. Pharmaceuticals (Basel). 2010. PMID: 27713280 Free PMC article. Review.
• Antiviral activity of limitin against encephalomyocarditis virus, herpes simplex virus, and mouse hepatitis virus: diverse requirements by limitin and alpha interferon for interferon regulatory factor 1.
  Kawamoto S, Oritani K, Asada H, Takahashi I, Ishikawa J, Yoshida H, Yamada M, Ishida N, Ujiie H, Masaie H, Tomiyama Y, Matsuzawa Y. Kawamoto S, et al. J Virol. 2003 Sep;77(17):9622-31. doi: 10.1128/jvi.77.17.9622-9631.2003. J Virol. 2003. PMID: 12915574 Free PMC article.
• Crystal structure of Zebrafish interferons I and II reveals conservation of type I interferon structure in vertebrates.
  Hamming OJ, Lutfalla G, Levraud JP, Hartmann R. Hamming OJ, et al. J Virol. 2011 Aug;85(16):8181-7. doi: 10.1128/JVI.00521-11. Epub 2011 Jun 8. J Virol. 2011. PMID: 21653665 Free PMC article.
• Generation of interleukin-6 receptor antagonists by molecular-modeling guided mutagenesis of residues important for gp130 activation.
  Savino R, Lahm A, Salvati AL, Ciapponi L, Sporeno E, Altamura S, Paonessa G, Toniatti C, Ciliberto G. Savino R, et al. EMBO J. 1994 Mar 15;13(6):1357-67. doi: 10.1002/j.1460-2075.1994.tb06389.x. EMBO J. 1994. PMID: 7511100 Free PMC article.

References

1. 1. Prog Nucleic Acid Res Mol Biol. 1986;33:251-300 - PubMed
2. 1. J Biol Chem. 1987 Apr 5;262(10):4804-5 - PubMed
3. 1. J Biol Chem. 1989 Oct 15;264(29):17595-605 - PubMed
4. 1. Proc Natl Acad Sci U S A. 1989 Dec;86(24):9667-71 - PubMed
5. 1. J Mol Biol. 1979 Jul 15;131(4):855-69 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • Nature Publishing Group
  • PubMed Central
  • Wiley

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • GlyGen glycoinformatics resource