Rorgamma (Rorc) is a common integration site in type B leukemogenic virus-induced T-cell lymphomas - PubMed (original) (raw)

Rorgamma (Rorc) is a common integration site in type B leukemogenic virus-induced T-cell lymphomas

Dana R Broussard et al. J Virol. 2004 May.

Abstract

The retrovirus type B leukemogenic virus (TBLV) causes T-cell lymphomas in mice. We have identified the Rorgamma locus as an integration site in 19% of TBLV-induced tumors. Overexpression of one or more Rorgamma isoforms in >77% of the tumors tested may complement apoptotic effects of c-myc overexpression.

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Figures

FIG. 1.

Location of TBLV insertions within the _Ror_γ locus in T-cell lymphomas as detected by PCR. Black arrows represent the location and orientation of TBLV proviruses. The tumors (designated T) containing the integrations are indicated closest to the arrow. Black bars represent _Ror_γ exons; 5′ and 3′ UTRs are indicated by hatched boxes.

FIG. 2.

Both c-myc and _Ror_γ/γt are overexpressed in the majority of TBLV-induced tumors. c-myc (black bars), _Ror_γt (gray bars), and _Ror_γ (white bars) expression levels from real-time RT-PCR analysis are shown relative to that from normal thymus. The standard errors for gene expression levels greater than 20-fold that of normal thymus are as follows: T13, _Ror_γ, 38 ± 0.4; T623B, c-myc, 27 ± 0.1; T703, c-myc, 22 ± 0.1; T708, c-myc, 21 ± 0.4; T708, _Ror_γt, 52 ± 0.2; T709, c-myc, 21 ± 0.3; C3H liver, _Ror_γ, 33 ± 0.02. Gene expression experiments were performed in triplicate three to five times depending on the availability of tumor RNA. Real-time RT-PCR primers were used at a final concentration of 0.1 to 0.2 μM and had been previously determined to have similar amplification efficiencies (slopes of <0.1).

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