Human immunodeficiency virus type 1 envelope glycoproteins that lack cytoplasmic domain cysteines: impact on association with membrane lipid rafts and incorporation onto budding virus particles - PubMed (original) (raw)
Human immunodeficiency virus type 1 envelope glycoproteins that lack cytoplasmic domain cysteines: impact on association with membrane lipid rafts and incorporation onto budding virus particles
Jayanta Bhattacharya et al. J Virol. 2004 May.
Abstract
The human immunodeficiency virus type 1 (HIV-1) envelope comprises a surface gp120 and a transmembrane gp41. The cytoplasmic domain of gp41 contains cysteine residues (C764 and C837) which are targets for palmitoylation and were reported to be required for envelope association with lipid rafts and assembly on budding virions (I. Rousso, M. B. Mixon, B. K. Chen, and P. S. Kim, Proc. Natl. Acad. Sci. USA 97:13523-13525, 2000). Several infectious HIV-1 clones contain envelopes that have no gp41 cytoplasmic cysteines. Since no other gp41 amino acid is a target for palmitoylation, these clones imply that palmitoylation is not essential for envelope trafficking and assembly. Here, we show that HIV-1 envelope mutants that lack gp41 cytoplasmic cysteines are excluded from light lipid rafts. Envelopes that contained residues with bulky hydrophobic side chains instead of cysteines retained their association with heavy rafts and were nearly fully functional for incorporation into virions and infectivity. Substitution of cysteines with alanines or serines eliminated raft association and more severely reduced envelope incorporation onto virions and their infectivity. Nevertheless, the A764/A837 mutant envelope retained nearly 40% infectivity compared to the wild type, even though this envelope was excluded from lipid rafts. Our results demonstrate that gp41 cytoplasmic cysteines that are targets for palmitoylation and are required for envelope trafficking to classical lipid rafts are not essential for HIV-1 replication.
Figures
FIG. 1.
The cytoplasmic domain of gp41. (A) LLP-1, LLP-2, and LLP-3 are putative α-helices that are proposed to interact with lipid bilayers. Palmitate groups covalently attached to C764 and C837 may insert into the lipid bilayer and anchor these regions to the plasma membrane. Palmitoylation may target gp41 into lipid rafts (21). (B) Amino acid substitutions introduced into the gp41 cytoplasmic domain.
FIG. 2.
Envelope expression and function. (A) 293T cells were cotransfected with pNL43env− and pSVIIIenv containing gp41 envelope mutants (C764/Y837 [CY], F764/Y837 [FY], A764/A837 [AA], and S764/S837 [SS]) and were immunostained for envelope. Cells were fixed and permeabilized with methanol-acetone (1:1) 48 h after transfection before immunostaining with anti-gp41 MAb Chessie 8 (1). (B) Cell surface envelope expression. 293T cells (transfected as described for panel A) were stained for surface envelope expression by using anti-gp120 MAb 902 (5, 19) and examined by flow cytometry. (C) Cell-cell fusion induced by NL43 mutant envelopes. 293T cells (transfected as described for panel A) were cocultivated with NP2/CD4/CXCR4. Cells were fixed and stained with 1% methylene blue-0.25% basic fuchsin in methanol.
FIG. 3.
Infectivity conferred by HIV-1 NL43 envelopes lacking gp41 cytoplasmic cysteines. Infectivity was tested with CD4+ indicator cell lines as shown. (A) GHOST parental cells that express endogenous CXCR4; (B) NP2/CD4/CXCR4 cells; (C) GHOST/CXCR4 cells. Cells were fixed 72 h after infection and immunostained for p24. Infectivity titers were then scored as focus-forming units (FFU) per picogram of RT in virus supernatants as measured by ELISA.
FIG. 4.
Incorporation of mutant NL43 envelopes onto virus particles. 293T cells were cotransfected with pNL43env− and pSVIIIenv containing gp41 envelope mutants (C764/Y837 [CY], F764/Y837 [FY], A764/A837 [AA], and S764/S837 [SS]). Supernatants harvested 48 h after transfection were spun at low speed and filtered through a 0.45-μm-pore-size syringe filter. The supernatants were then ultracentrifuged at 100,000 × g for 2 h at 4°C to pellet virus particles. The pelleted virions were resuspended and measured for RT activity. Equivalent amounts of RT activity were then resolved on a sodium dodecyl sulfate-8% polyacrylamide gel electrophoresis gel followed by Western blotting with anti-gp41 Chessie 8 or anti-p24 183-H12-5C as primary antibodies. (A) Western blot analysis of envelope on virions; (B) ratios of envelope to p24 gag concentration evaluated by densitometry of Western blot bands.
FIG.5.
Association of mutant NL43 envelopes with lipid rafts. 293T cells were cotransfected with pNL43env− and pSVIIIenv containing gp41 envelope mutants. Cells were lysed with 0.5% Triton X-100 in TNE buffer on ice. Lysates were homogenized, cleared of nuclei, adjusted to 60% sucrose, and applied to the bottom of a sucrose gradient (see text). Gradient fractions were Western blotted and probed for envelope and gag with mouse MAbs. (A) Sucrose gradient fractionation of cold Triton X-100 extracts from 293T cells cotransfected with pNL43env− and pSVIIIenv encoding the NL43 envelope mutants C764/Y837 (CY), F764/Y837 (FY), A764/A837 (AA), and S764/S837 (SS). Note that the NL43 CY envelope associates with both the DRM-L and the DRM-H fractions. FY is excluded from DRM-L but retains an association with DRM-H. The AA and SS envelopes are completely excluded from rafts. (B) Sucrose gradient fractionations, as described for panel A except that Triton X-100 extractions were carried out at 37°C to solubilize lipid rafts. Note that all the gag precursor and envelope detected is present in the fractions representing soluble membranes and not at densities that correspond to lipid rafts. (C) Sucrose gradient of 293T cold Triton X-100 extracts, blotted and probed with a rabbit polyclonal antibody to caveolin (molecular mass, 20 to 22 kDa; DRM-L marker) (BD Biosciences Transduction Laboratories Inc.).
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References
- Abacioglu, Y. H., T. R. Fouts, J. D. Laman, E. Claassen, S. H. Pincus, J. P. Moore, C. A. Roby, R. Kamin-Lewis, and G. K. Lewis. 1994. Epitope mapping and topology of baculovirus-expressed HIV-1 gp160 determined with a panel of murine monoclonal antibodies. AIDS Res. Hum. Retrovir. 10:371-381. - PubMed
- Brown, D. 2002. Structure and function of membrane rafts. Int. J. Med. Microbiol. 291:433-437. - PubMed
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