Activity-dependent phosphorylation of tyrosine hydroxylase in dopaminergic neurons of the rat retina - PubMed (original) (raw)

Effect of TTX on TH-P immunoreactivity. Left-right pairs (a/b, etc.) show identical fields immunostained with anti-panTH (left member of the pair) and one of three anti-phosphoTH antibodies, as labeled. a/b, e/f, i/j, Left eye injected in darkness with bicuculline (100 μ

m

) and strychnine (20 μ

m

). c/d, g/h, k/l, Right eye of same rat injected in darkness with bicuculline, strychnine, and TTX (15 μ

m

). Bicuculline plus strychnine alone induced THP in DA cell bodies, dendrites, and DA plexus (b, f, j). In the presence of TTX, bicuculline, plus strychnine, THP is weak or absent in the DA plexus (d, h, l) but is present in DA cell bodies and dendrites. TTX did not affect anti-panTH-IR (compare a,e,i with c,g,k). Scale bar in i applies to all panels. gly, Glycine.