Protein displacement by DExH/D "RNA helicases" without duplex unwinding - PubMed (original) (raw)
. 2004 Apr 30;304(5671):730-4.
doi: 10.1126/science.1095596.
Affiliations
- PMID: 15118161
- DOI: 10.1126/science.1095596
Protein displacement by DExH/D "RNA helicases" without duplex unwinding
Margaret E Fairman et al. Science. 2004.
Abstract
Members of the DExH/D superfamily of nucleic acid-activated nucleotide triphosphatases are essential for virtually all aspects of RNA metabolism, including pre-messenger RNA splicing, RNA interference, translation, and nucleocytoplasmic trafficking. Physiological substrates for these enzymes are thought to be regions of double-stranded RNA, because several DExH/D proteins catalyze strand separation in vitro. These "RNA helicases" can also disrupt RNA-protein interactions, but it is unclear whether this activity is coupled to duplex unwinding. Here we demonstrate that two unrelated DExH/D proteins catalyze protein displacement independently of duplex unwinding. Therefore, the essential functions of DExH/D proteins are not confined to RNA duplexes but can be exerted on a wide range of ribonucleoprotein substrates.
Comment in
- Molecular biology. The life of RNA with proteins.
Linder P. Linder P. Science. 2004 Apr 30;304(5671):694-5. doi: 10.1126/science.1097850. Science. 2004. PMID: 15118153 No abstract available.
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