Stability and inactivation of SARS coronavirus - PubMed (original) (raw)
Stability and inactivation of SARS coronavirus
H F Rabenau et al. Med Microbiol Immunol. 2005 Jan.
Abstract
The SARS-coronavirus (SARS-CoV) is a newly emerged, highly pathogenic agent that caused over 8,000 human infections with nearly 800 deaths between November 2002 and September 2003. While direct person-to-person transmission via respiratory droplets accounted for most cases, other modes have not been ruled out. Faecal shedding is common and prolonged and has caused an outbreak in Hong Kong. We studied the stability of SARS-CoV under different conditions, both in suspension and dried on surfaces, in comparison with other human-pathogenic viruses, including human coronavirus HCoV-229E. In suspension, HCoV-229E gradually lost its infectivity completely while SARS-CoV retained its infectivity for up to 9 days; in the dried state, survival times were 24 h versus 6 days. Thermal inactivation at 56 degrees C was highly effective in the absence of protein, reducing the virus titre to below detectability; however, the addition of 20% protein exerted a protective effect resulting in residual infectivity. If protein-containing solutions are to be inactivated, heat treatment at 60 degrees C for at least 30 min must be used. Different fixation procedures, e.g. for the preparation of immunofluorescence slides, as well as chemical means of virus inactivation commonly used in hospital and laboratory settings were generally found to be effective. Our investigations confirm that it is possible to care for SARS patients and to conduct laboratory scientific studies on SARS-CoV safely. Nevertheless, the agents tenacity is considerably higher than that of HCoV-229E, and should SARS re-emerge, increased efforts need to be devoted to questions of environmental hygiene.
Figures
Fig. 1A–C
SARS-CoV replication in Vero cells determined by immune peroxidase staining using serum from the index patient. Infected cells were stained 24 h (A) and 48 h (B) post-infection. Mock-infected cells are also shown (C)
Fig. 2a–d
In vitro stability of SARS-CoV, HCoV-229E, HSV-1 and adenovirus type 3 either in suspension or dried. Infected cell culture supernatants were incubated at RT either in suspension (c, d) or dried on a plastic surface (a, b), in the presence (b, d) or absence (a, c) of 10% FCS. Values are means from three independent experiments. The SD did not exceed 20%. ▲ SARS-CoV (FFM1), ♦ h-CoV (E229), ■ HSV-1, ● adenovirus type 3, ⋅⋅⋅⋅⋅⋅ detection limit
References
- Centers Morb Mortal Wkly Rep Surveill Summ. 2003;52:241.
- Department of Communicable Disease Surveillance and Response (2003) Consensus document on the epidemiology of severe acute respiratory syndrome (SARS). WHO/CDS/CSR/GAR/2003.11. WHO, Geneva
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