Intraspinal transplantation of CD34+ human umbilical cord blood cells after spinal cord hemisection injury improves functional recovery in adult rats - PubMed (original) (raw)
Intraspinal transplantation of CD34+ human umbilical cord blood cells after spinal cord hemisection injury improves functional recovery in adult rats
Zong Mao Zhao et al. Cell Transplant. 2004.
Free article
Abstract
The present study was designed to compare the functional outcome of the intraspinal transplantation of CD34+ human umbilical cord blood (CB) cells with that of human bone marrow stromal (BMS) cells in adult rats with spinal cord injury. Sixty adult Wistar rats were subjected to left spinal cord hemisection, and then divided into three groups randomly. The control group received an injection of PBS without cells, while the two other groups of rats received a transplantation of 5 x 10(5) CD34+ CB or BMS cells, respectively. Functional outcome was measured using the modified Tarlov score at days 1, 7, 14, 21, and 28 after transplantation. A statistically significant improvement in functional outcome and survival rate in the experimental groups of rats was observed compared with the control group. Rats that received CD34+ CB cells achieved a better improvement in functional score than those that received BMS cells at days 7 and 14 after transplantation. Histological evaluation revealed that bromodeoxyuridine (BrdU)-labeled CD34+ CB and BMS cells survived and migrated into the injured area. Some of these cells expressed glial fibriliary acidic protein (GFAP) or neuronal nuclear antigen (NeuN). Our data demonstrate for the first time that intraspinal transplantation of human CD34+ CB cells provides benefit in function recovery after spinal cord hemisection in rats and suggest that CD34+ CB cells may be an excellent choice of cells as routine starting material of allogenic and autologous transplantations for the treatment of spinal cord injury.
Similar articles
- [Transplantation of human umbilical cord stem cells improves neurological function recovery after spinal cord injury in rats].
Li HJ, Liu HY, Zhao ZM, Lu SH, Yang RC, Zhu HF, Cai YL, Zhang QJ, Han ZC. Li HJ, et al. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2004 Feb;26(1):38-42. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2004. PMID: 15052772 Chinese. - The use of hemopoietic stem cells derived from human umbilical cord blood to promote restoration of spinal cord tissue and recovery of hindlimb function in adult rats.
Nishio Y, Koda M, Kamada T, Someya Y, Yoshinaga K, Okada S, Harada H, Okawa A, Moriya H, Yamazaki M. Nishio Y, et al. J Neurosurg Spine. 2006 Nov;5(5):424-33. doi: 10.3171/spi.2006.5.5.424. J Neurosurg Spine. 2006. PMID: 17120892 - Transplantation of bone marrow stromal cells for treatment of central nervous system diseases.
Chopp M, Li Y. Chopp M, et al. Adv Exp Med Biol. 2006;585:49-64. doi: 10.1007/978-0-387-34133-0_4. Adv Exp Med Biol. 2006. PMID: 17120776 Review. No abstract available. - Human umbilical cord blood stem cells: rational for use as a neuroprotectant in ischemic brain disease.
Arien-Zakay H, Lecht S, Nagler A, Lazarovici P. Arien-Zakay H, et al. Int J Mol Sci. 2010 Sep 21;11(9):3513-28. doi: 10.3390/ijms11093513. Int J Mol Sci. 2010. PMID: 20957109 Free PMC article. Review.
Cited by
- Therapeutic potentials of mesenchymal stem cells derived from human umbilical cord.
Fan CG, Zhang QJ, Zhou JR. Fan CG, et al. Stem Cell Rev Rep. 2011 Mar;7(1):195-207. doi: 10.1007/s12015-010-9168-8. Stem Cell Rev Rep. 2011. PMID: 20676943 Review. - The great migration of bone marrow-derived stem cells toward the ischemic brain: therapeutic implications for stroke and other neurological disorders.
Borlongan CV, Glover LE, Tajiri N, Kaneko Y, Freeman TB. Borlongan CV, et al. Prog Neurobiol. 2011 Oct;95(2):213-28. doi: 10.1016/j.pneurobio.2011.08.005. Epub 2011 Aug 30. Prog Neurobiol. 2011. PMID: 21903148 Free PMC article. Review. - Transplantation of umbilical cord blood stem cells for treating spinal cord injury.
Park DH, Lee JH, Borlongan CV, Sanberg PR, Chung YG, Cho TH. Park DH, et al. Stem Cell Rev Rep. 2011 Mar;7(1):181-94. doi: 10.1007/s12015-010-9163-0. Stem Cell Rev Rep. 2011. PMID: 20532836 Review. - Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs.
Lim JH, Byeon YE, Ryu HH, Jeong YH, Lee YW, Kim WH, Kang KS, Kweon OK. Lim JH, et al. J Vet Sci. 2007 Sep;8(3):275-82. doi: 10.4142/jvs.2007.8.3.275. J Vet Sci. 2007. PMID: 17679775 Free PMC article. - Non-hematopoietic stem cells in umbilical cord blood.
Matsumoto T, Mugishima H. Matsumoto T, et al. Int J Stem Cells. 2009 May;2(2):83-9. doi: 10.15283/ijsc.2009.2.2.83. Int J Stem Cells. 2009. PMID: 24855525 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Miscellaneous