Bidirectional role of orphan nuclear receptor RORalpha in clock gene transcriptions demonstrated by a novel reporter assay system - PubMed (original) (raw)

Bidirectional role of orphan nuclear receptor RORalpha in clock gene transcriptions demonstrated by a novel reporter assay system

Yoshihiro Nakajima et al. FEBS Lett. 2004.

Free article

Abstract

Circadian rhythms are generated by an extremely complicated transcription-translation feedback loop. To precisely analyze the molecular mechanisms of the circadian clock, it is critical to monitor multiple gene expressions and/or interactions with their transcription factors simultaneously. We have developed a novel reporter assay system, the tricolor reporter in vitro assay system, which consists of green- and red-emitting Phrixothrix luciferases as dual reporters and blue-emitting Renilla luciferase as internal control. We have successfully employed this system in analyzing the effects of clock gene products on the enhancer elements of Per1 and Bmal1 promoters. The results indicate that the orphan nuclear receptor RORalpha regulates bidirectionally Bmal1 (positively) and Per1 (negatively) transcriptions simultaneously.

PubMed Disclaimer

Similar articles

• The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1.
  Akashi M, Takumi T. Akashi M, et al. Nat Struct Mol Biol. 2005 May;12(5):441-8. doi: 10.1038/nsmb925. Epub 2005 Apr 10. Nat Struct Mol Biol. 2005. PMID: 15821743
• Synergistic regulation of the mouse orphan nuclear receptor SHP gene promoter by CLOCK-BMAL1 and LRH-1.
  Oiwa A, Kakizawa T, Miyamoto T, Yamashita K, Jiang W, Takeda T, Suzuki S, Hashizume K. Oiwa A, et al. Biochem Biophys Res Commun. 2007 Feb 23;353(4):895-901. doi: 10.1016/j.bbrc.2006.12.131. Epub 2006 Dec 26. Biochem Biophys Res Commun. 2007. PMID: 17204240
• Expression of the gene for Dec2, a basic helix-loop-helix transcription factor, is regulated by a molecular clock system.
  Hamaguchi H, Fujimoto K, Kawamoto T, Noshiro M, Maemura K, Takeda N, Nagai R, Furukawa M, Honma S, Honma K, Kurihara H, Kato Y. Hamaguchi H, et al. Biochem J. 2004 Aug 15;382(Pt 1):43-50. doi: 10.1042/BJ20031760. Biochem J. 2004. PMID: 15147242 Free PMC article.
• A rhythmic Ror.
  Emery P, Reppert SM. Emery P, et al. Neuron. 2004 Aug 19;43(4):443-6. doi: 10.1016/j.neuron.2004.08.009. Neuron. 2004. PMID: 15312644 Review.
• [A systems-biological approach in drug discovery for circadian rhythm disorders].
  Ueda HR. Ueda HR. Nihon Yakurigaku Zasshi. 2002 Nov;120(1):37P-40P. Nihon Yakurigaku Zasshi. 2002. PMID: 12491774 Review. Japanese.

Cited by

• The Absence of Pineal Melatonin Abolishes the Daily Rhythm of Tph1 (Tryptophan Hydroxylase 1), Asmt (Acetylserotonin O-Methyltransferase), and Aanat (Aralkylamine N-Acetyltransferase) mRNA Expressions in Rat Testes.
  Coelho LA, Andrade-Silva J, Motta-Teixeira LC, Amaral FG, Reiter RJ, Cipolla-Neto J. Coelho LA, et al. Mol Neurobiol. 2019 Nov;56(11):7800-7809. doi: 10.1007/s12035-019-1626-y. Epub 2019 May 23. Mol Neurobiol. 2019. PMID: 31124080
• The clock gene PER1 plays an important role in regulating the clock gene network in human oral squamous cell carcinoma cells.
  Zhao Q, Zheng G, Yang K, Ao YR, Su XL, Li Y, Lv XQ. Zhao Q, et al. Oncotarget. 2016 Oct 25;7(43):70290-70302. doi: 10.18632/oncotarget.11844. Oncotarget. 2016. PMID: 27602964 Free PMC article.
• Differential roles of AVP and VIP signaling in the postnatal changes of neural networks for coherent circadian rhythms in the SCN.
  Ono D, Honma S, Honma K. Ono D, et al. Sci Adv. 2016 Sep 9;2(9):e1600960. doi: 10.1126/sciadv.1600960. eCollection 2016 Sep. Sci Adv. 2016. PMID: 27626074 Free PMC article.
• Retinoid-related Orphan Receptors (RORs): Roles in Cellular Differentiation and Development.
  Jetten AM, Joo JH. Jetten AM, et al. Adv Dev Biol. 2006;16:313-355. doi: 10.1016/S1574-3349(06)16010-X. Adv Dev Biol. 2006. PMID: 18418469 Free PMC article.
• Circadian Rhythm Alteration of the Core Clock Genes and the Lipid Metabolism Genes Induced by High-Fat Diet (HFD) in the Liver Tissue of the Chinese Soft-Shelled Turtle (Trionyx sinensis).
  Liu L, Liu L, Deng S, Zou L, He Y, Zhu X, Li H, Hu Y, Chu W, Wang X. Liu L, et al. Genes (Basel). 2024 Jan 25;15(2):157. doi: 10.3390/genes15020157. Genes (Basel). 2024. PMID: 38397147 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science
  • Wiley

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • Guide to Pharmacology