Real-time imaging of peroxisome proliferator-activated receptor-gamma coactivator-1alpha promoter activity in skeletal muscles of living mice - PubMed (original) (raw)

. 2004 Sep;287(3):C790-6.

doi: 10.1152/ajpcell.00425.2003. Epub 2004 May 19.

Affiliations

• PMID: 15151904
• DOI: 10.1152/ajpcell.00425.2003

Free article

Real-time imaging of peroxisome proliferator-activated receptor-gamma coactivator-1alpha promoter activity in skeletal muscles of living mice

Takayuki Akimoto et al. Am J Physiol Cell Physiol. 2004 Sep.

Free article

Abstract

In response to sustained increase in contractile activity, mammalian skeletal muscle undergoes adaptation with enhanced mitochondrial biogenesis and fiber type switching. The peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) was recently identified as a key regulator for these adaptive processes. To investigate the sequence elements in the PGC-1alpha gene that are responsible for activity-dependent transcriptional activation, we have established a unique system to analyze promoter activity in skeletal muscle of living mice. Expression of PGC-1alpha-firefly luciferase reporter gene in mouse tibialis anterior muscle transfected by electric pulse-mediated gene transfer was assessed repeatedly in the same muscle by using optical bioluminescence imaging analysis before and after low-frequency (10 Hz) motor nerve stimulation. Nerve stimulation (2 h) resulted in a transient 3-fold increase (P < 0.05) in PGC-1alpha promoter activity along with a 1.6-fold increase (P < 0.05) in endogenous PGC-1alpha mRNA. Mutation of two consensus myocyte enhancer factor 2 (MEF2) binding sites (-2901 and -1539) or a cAMP response element (CRE) (-222) completely abolished nerve stimulation-induced increase in PGC-1alpha promoter activity. These findings provide direct evidence that contractile activity-induced PGC-1alpha promoter activity in skeletal muscle is dependent on the MEF2 and the CRE sequence elements. The experimental methods used in the present study have general applicability to studies of gene regulation in muscle.

PubMed Disclaimer

Similar articles

• Exercise stimulates Pgc-1alpha transcription in skeletal muscle through activation of the p38 MAPK pathway.
  Akimoto T, Pohnert SC, Li P, Zhang M, Gumbs C, Rosenberg PB, Williams RS, Yan Z. Akimoto T, et al. J Biol Chem. 2005 May 20;280(20):19587-93. doi: 10.1074/jbc.M408862200. Epub 2005 Mar 14. J Biol Chem. 2005. PMID: 15767263
• Functional interaction of regulatory factors with the Pgc-1alpha promoter in response to exercise by in vivo imaging.
  Akimoto T, Li P, Yan Z. Akimoto T, et al. Am J Physiol Cell Physiol. 2008 Jul;295(1):C288-92. doi: 10.1152/ajpcell.00104.2008. Epub 2008 Apr 23. Am J Physiol Cell Physiol. 2008. PMID: 18434626 Free PMC article.
• SIRT1 controls the transcription of the peroxisome proliferator-activated receptor-gamma Co-activator-1alpha (PGC-1alpha) gene in skeletal muscle through the PGC-1alpha autoregulatory loop and interaction with MyoD.
  Amat R, Planavila A, Chen SL, Iglesias R, Giralt M, Villarroya F. Amat R, et al. J Biol Chem. 2009 Aug 14;284(33):21872-21880. doi: 10.1074/jbc.M109.022749. Epub 2009 Jun 24. J Biol Chem. 2009. PMID: 19553684 Free PMC article.
• Adaptation of Skeletal Muscles to Contractile Activity of Varying Duration and Intensity: The Role of PGC-1α.
  Popov DV. Popov DV. Biochemistry (Mosc). 2018 Jun;83(6):613-628. doi: 10.1134/S0006297918060019. Biochemistry (Mosc). 2018. PMID: 30195320 Review.
• PGC-1alpha-mediated adaptations in skeletal muscle.
  Olesen J, Kiilerich K, Pilegaard H. Olesen J, et al. Pflugers Arch. 2010 Jun;460(1):153-62. doi: 10.1007/s00424-010-0834-0. Epub 2010 Apr 19. Pflugers Arch. 2010. PMID: 20401754 Review.

Cited by

• Marine-derived Compounds: A Powerful Platform for the Treatment of Alzheimer's Disease.
  Arora R, Babbar R, Dabra A, Chopra B, Deswal G, Grewal AS. Arora R, et al. Cent Nerv Syst Agents Med Chem. 2024;24(2):166-181. doi: 10.2174/0118715249269050231129103002. Cent Nerv Syst Agents Med Chem. 2024. PMID: 38305396 Review.
• The impact of diet upon mitochondrial physiology (Review).
  Kyriazis ID, Vassi E, Alvanou M, Angelakis C, Skaperda Z, Tekos F, Garikipati VNS, Spandidos DA, Kouretas D. Kyriazis ID, et al. Int J Mol Med. 2022 Nov;50(5):135. doi: 10.3892/ijmm.2022.5191. Epub 2022 Sep 21. Int J Mol Med. 2022. PMID: 36129147 Free PMC article. Review.
• Salvianolic Acid B Alleviates Limb Ischemia in Mice via Promoting SIRT1/PI3K/AKT Pathway-Mediated M2 Macrophage Polarization.
  Niu W, Wu F, Cao W, Chen Y, Zhang Y, Chen Y, Ding R, Liang C. Niu W, et al. Evid Based Complement Alternat Med. 2022 May 24;2022:1112394. doi: 10.1155/2022/1112394. eCollection 2022. Evid Based Complement Alternat Med. 2022. PMID: 35656466 Free PMC article.
• CORP: Gene delivery into murine skeletal muscle using in vivo electroporation.
  Hughes DC, Hardee JP, Waddell DS, Goodman CA. Hughes DC, et al. J Appl Physiol (1985). 2022 Jul 1;133(1):41-59. doi: 10.1152/japplphysiol.00088.2022. Epub 2022 May 5. J Appl Physiol (1985). 2022. PMID: 35511722 Free PMC article. Review.
• Serine-to-glycine ratios in low-protein diets regulate intramuscular fat by affecting lipid metabolism and myofiber type transition in the skeletal muscle of growing-finishing pigs.
  Zhou X, Liu Y, Zhang L, Kong X, Li F. Zhou X, et al. Anim Nutr. 2021 Jun;7(2):384-392. doi: 10.1016/j.aninu.2020.08.011. Epub 2021 Mar 5. Anim Nutr. 2021. PMID: 34258426 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon