Differential stability of beta-catenin along the animal-vegetal axis of the sea urchin embryo mediated by dishevelled - PubMed (original) (raw)
. 2004 Jun;131(12):2947-56.
doi: 10.1242/dev.01152. Epub 2004 May 19.
Affiliations
- PMID: 15151983
- DOI: 10.1242/dev.01152
Differential stability of beta-catenin along the animal-vegetal axis of the sea urchin embryo mediated by dishevelled
Heather E Weitzel et al. Development. 2004 Jun.
Abstract
beta-Catenin has a central role in the early axial patterning of metazoan embryos. In the sea urchin, beta-catenin accumulates in the nuclei of vegetal blastomeres and controls endomesoderm specification. Here, we use in-vivo measurements of the half-life of fluorescently tagged beta-catenin in specific blastomeres to demonstrate a gradient in beta-catenin stability along the animal-vegetal axis during early cleavage. This gradient is dependent on GSK3beta-mediated phosphorylation of beta-catenin. Calculations show that the difference in beta-catenin half-life at the animal and vegetal poles of the early embryo is sufficient to produce a difference of more than 100-fold in levels of the protein in less than 2 hours. We show that dishevelled (Dsh), a key signaling protein, is required for the stabilization of beta-catenin in vegetal cells and provide evidence that Dsh undergoes a local activation in the vegetal region of the embryo. Finally, we report that GFP-tagged Dsh is targeted specifically to the vegetal cortex of the fertilized egg. During cleavage, Dsh-GFP is partitioned predominantly into vegetal blastomeres. An extensive mutational analysis of Dsh identifies several regions of the protein that are required for vegetal cortical targeting, including a phospholipid-binding motif near the N-terminus.
Similar articles
- The emergence of pattern in embryogenesis: regulation of beta-catenin localization during early sea urchin development.
Ettensohn CA. Ettensohn CA. Sci STKE. 2006 Nov 14;2006(361):pe48. doi: 10.1126/stke.3612006pe48. Sci STKE. 2006. PMID: 17106077 Review. - Differential regulation of disheveled in a novel vegetal cortical domain in sea urchin eggs and embryos: implications for the localized activation of canonical Wnt signaling.
Peng CJ, Wikramanayake AH. Peng CJ, et al. PLoS One. 2013 Nov 13;8(11):e80693. doi: 10.1371/journal.pone.0080693. eCollection 2013. PLoS One. 2013. PMID: 24236196 Free PMC article. - Analysis of dishevelled localization and function in the early sea urchin embryo.
Leonard JD, Ettensohn CA. Leonard JD, et al. Dev Biol. 2007 Jun 1;306(1):50-65. doi: 10.1016/j.ydbio.2007.02.041. Epub 2007 Mar 6. Dev Biol. 2007. PMID: 17433285 Free PMC article. - beta-Catenin is essential for patterning the maternally specified animal-vegetal axis in the sea urchin embryo.
Wikramanayake AH, Huang L, Klein WH. Wikramanayake AH, et al. Proc Natl Acad Sci U S A. 1998 Aug 4;95(16):9343-8. doi: 10.1073/pnas.95.16.9343. Proc Natl Acad Sci U S A. 1998. PMID: 9689082 Free PMC article. - Animal-vegetal axis patterning mechanisms in the early sea urchin embryo.
Angerer LM, Angerer RC. Angerer LM, et al. Dev Biol. 2000 Feb 1;218(1):1-12. doi: 10.1006/dbio.1999.9553. Dev Biol. 2000. PMID: 10644406 Review.
Cited by
- Evolutionarily conserved Wnt/Sp5 signaling is critical for anterior-posterior axis patterning in sea urchin embryos.
Gautam S, Fenner JL, Wang B, Range RC. Gautam S, et al. iScience. 2023 Dec 2;27(1):108616. doi: 10.1016/j.isci.2023.108616. eCollection 2024 Jan 19. iScience. 2023. PMID: 38179064 Free PMC article. - microRNA-124 directly suppresses Nodal and Notch to regulate mesodermal development.
Konrad KD, Arnott M, Testa M, Suarez S, Song JL. Konrad KD, et al. Dev Biol. 2023 Oct;502:50-62. doi: 10.1016/j.ydbio.2023.06.017. Epub 2023 Jul 5. Dev Biol. 2023. PMID: 37419400 Free PMC article. - Live imaging of echinoderm embryos to illuminate evo-devo.
Barone V, Lyons DC. Barone V, et al. Front Cell Dev Biol. 2022 Sep 15;10:1007775. doi: 10.3389/fcell.2022.1007775. eCollection 2022. Front Cell Dev Biol. 2022. PMID: 36187474 Free PMC article. - Architecture and evolution of the _cis_-regulatory system of the echinoderm kirrelL gene.
Khor JM, Ettensohn CA. Khor JM, et al. Elife. 2022 Feb 25;11:e72834. doi: 10.7554/eLife.72834. Elife. 2022. PMID: 35212624 Free PMC article. - Micromere formation and its evolutionary implications in the sea urchin.
Emura N, Yajima M. Emura N, et al. Curr Top Dev Biol. 2022;146:211-238. doi: 10.1016/bs.ctdb.2021.10.008. Epub 2021 Dec 3. Curr Top Dev Biol. 2022. PMID: 35152984 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous