Transforming a (beta/alpha)8--barrel enzyme into a split-protein sensor through directed evolution - PubMed (original) (raw)

Programmed evolution for optimization of orthogonal metabolic output in bacteria.

Eckdahl TT, Campbell AM, Heyer LJ, Poet JL, Blauch DN, Snyder NL, Atchley DT, Baker EJ, Brown M, Brunner EC, Callen SA, Campbell JS, Carr CJ, Carr DR, Chadinha SA, Chester GI, Chester J, Clarkson BR, Cochran KE, Doherty SE, Doyle C, Dwyer S, Edlin LM, Evans RA, Fluharty T, Frederick J, Galeota-Sprung J, Gammon BL, Grieshaber B, Gronniger J, Gutteridge K, Henningsen J, Isom B, Itell HL, Keffeler EC, Lantz AJ, Lim JN, McGuire EP, Moore AK, Morton J, Nakano M, Pearson SA, Perkins V, Parrish P, Pierson CE, Polpityaarachchige S, Quaney MJ, Slattery A, Smith KE, Spell J, Spencer M, Taye T, Trueblood K, Vrana CJ, Whitesides ET. Eckdahl TT, et al. PLoS One. 2015 Feb 25;10(2):e0118322. doi: 10.1371/journal.pone.0118322. eCollection 2015. PLoS One. 2015. PMID: 25714374 Free PMC article.