Amphiphysin is a component of clathrin coats formed during synaptic vesicle recycling at the lamprey giant synapse - PubMed (original) (raw)
Amphiphysin is a component of clathrin coats formed during synaptic vesicle recycling at the lamprey giant synapse
Emma Evergren et al. Traffic. 2004 Jul.
Free article
Abstract
Amphiphysin is a protein enriched at mammalian synapses thought to function as a clathrin accessory factor in synaptic vesicle endocytosis. Here we examine the involvement of amphiphysin in synaptic vesicle recycling at the giant synapse in the lamprey. We show that amphiphysin resides in the synaptic vesicle cluster at rest and relocates to sites of endocytosis during synaptic activity. It accumulates at coated pits where its SH3 domain, but not its central clathrin/AP-2-binding (CLAP) region, is accessible for antibody binding. Microinjection of antibodies specifically directed against the CLAP region inhibited recycling of synaptic vesicles and caused accumulation of clathrin-coated intermediates with distorted morphology, including flat patches of coated presynaptic membrane. Our data provide evidence for an activity-dependent redistribution of amphiphysin in intact nerve terminals and show that amphiphysin is a component of presynaptic clathrin-coated intermediates formed during synaptic vesicle recycling.
Similar articles
- A pre-embedding immunogold approach for detection of synaptic endocytic proteins in situ.
Evergren E, Tomilin N, Vasylieva E, Sergeeva V, Bloom O, Gad H, Capani F, Shupliakov O. Evergren E, et al. J Neurosci Methods. 2004 May 30;135(1-2):169-74. doi: 10.1016/j.jneumeth.2003.12.010. J Neurosci Methods. 2004. PMID: 15020101 - Selective perturbation of the BAR domain of endophilin impairs synaptic vesicle endocytosis.
Andersson F, Löw P, Brodin L. Andersson F, et al. Synapse. 2010 Jul;64(7):556-60. doi: 10.1002/syn.20772. Synapse. 2010. PMID: 20222153 - Synaptic vesicle endocytosis impaired by disruption of dynamin-SH3 domain interactions.
Shupliakov O, Löw P, Grabs D, Gad H, Chen H, David C, Takei K, De Camilli P, Brodin L. Shupliakov O, et al. Science. 1997 Apr 11;276(5310):259-63. doi: 10.1126/science.276.5310.259. Science. 1997. PMID: 9092476 - Giant reticulospinal synapse in lamprey: molecular links between active and periactive zones.
Brodin L, Shupliakov O. Brodin L, et al. Cell Tissue Res. 2006 Nov;326(2):301-10. doi: 10.1007/s00441-006-0216-2. Epub 2006 Jun 20. Cell Tissue Res. 2006. PMID: 16786368 Review. - Clathrin and synaptic vesicle endocytosis: studies at the squid giant synapse.
Augustine GJ, Morgan JR, Villalba-Galea CA, Jin S, Prasad K, Lafer EM. Augustine GJ, et al. Biochem Soc Trans. 2006 Feb;34(Pt 1):68-72. doi: 10.1042/BST0340068. Biochem Soc Trans. 2006. PMID: 16417485 Free PMC article. Review.
Cited by
- Genetically encoded intrabodies as high-precision tools to visualize and manipulate neuronal function.
Trimmer JS. Trimmer JS. Semin Cell Dev Biol. 2022 Jun;126:117-124. doi: 10.1016/j.semcdb.2021.11.004. Epub 2021 Nov 12. Semin Cell Dev Biol. 2022. PMID: 34782184 Free PMC article. - Synaptic vesicle exocytosis and increased cytosolic calcium are both necessary but not sufficient for activity-dependent bulk endocytosis.
Morton A, Marland JR, Cousin MA. Morton A, et al. J Neurochem. 2015 Aug;134(3):405-15. doi: 10.1111/jnc.13132. Epub 2015 May 14. J Neurochem. 2015. PMID: 25913068 Free PMC article. - α-Synuclein in the Synaptic Vesicle Liquid Phase: Active Player or Passive Bystander?
Brodin L, Milovanovic D, Rizzoli SO, Shupliakov O. Brodin L, et al. Front Mol Biosci. 2022 May 18;9:891508. doi: 10.3389/fmolb.2022.891508. eCollection 2022. Front Mol Biosci. 2022. PMID: 35664678 Free PMC article. Review. - Soluble membrane trafficking proteins taking a break at silent synaptic vesicles.
Brodin L, Gregory JA, Ackermann F. Brodin L, et al. Proc Natl Acad Sci U S A. 2011 Oct 11;108(41):16869-70. doi: 10.1073/pnas.1113641108. Epub 2011 Sep 23. Proc Natl Acad Sci U S A. 2011. PMID: 21949251 Free PMC article. No abstract available. - Anti-amphiphysin encephalitis: Expanding the clinical spectrum.
Sun Y, Qin X, Huang D, Zhou Z, Zhang Y, Wang Q. Sun Y, et al. Front Immunol. 2023 Apr 5;14:1084883. doi: 10.3389/fimmu.2023.1084883. eCollection 2023. Front Immunol. 2023. PMID: 37090693 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials