Regulated production of a peroxisome proliferator-activated receptor-gamma ligand during an early phase of adipocyte differentiation in 3T3-L1 adipocytes - PubMed (original) (raw)
. 2004 Aug 20;279(34):36093-102.
doi: 10.1074/jbc.M405346200. Epub 2004 Jun 9.
Affiliations
- PMID: 15190061
- DOI: 10.1074/jbc.M405346200
Free article
Regulated production of a peroxisome proliferator-activated receptor-gamma ligand during an early phase of adipocyte differentiation in 3T3-L1 adipocytes
Iphigenia Tzameli et al. J Biol Chem. 2004.
Free article
Abstract
Peroxisome proliferator-activated receptor-gamma (PPARgamma) is a nuclear hormone receptor that is critical for adipogenesis and insulin sensitivity. Ligands for PPARgamma include some polyunsaturated fatty acids and prostanoids and the synthetic high affinity antidiabetic agents thiazolidinediones. However, the identity of a biologically relevant endogenous PPARgamma ligand is unknown, and limited insight exists into the factors that may regulate production of endogenous PPARgamma ligands during adipocyte development. To address this question, we created a line of 3T3-L1 preadipocytes that carry a beta-galactosidase-based PPARgamma ligand-sensing vector system. In this system, induction of adipogenesis resulted in elevated beta-galactosidase activity that signifies activation of PPARgamma via its ligand-binding domain (LBD) and suggests generation and/or accumulation of a ligand moiety. The putative endogenous ligand appeared early in adipogenesis in response to increases in cAMP, accumulated in the medium, and dissipated later in adipogenesis. Organically extracted and high pressure liquid chromatography-fractionated conditioned media from differentiating cells, but not from mature adipocytes, were enriched in this activity. One or more components within the organic extract activated PPARgamma through interaction with its LBD, induced lipid accumulation in 3T3-L1 cells as efficiently as the differentiation mixture, and competed for binding of rosiglitazone to the LBD of PPARgamma. The active species appears to be different from other PPARgamma ligands identified previously. Our findings suggest that a novel biologically relevant PPARgamma ligand is transiently produced in 3T3-L1 cells during adipogenesis.
Similar articles
- Altering PPARgamma ligand selectivity impairs adipogenesis by thiazolidinediones but not hormonal inducers.
Samarasinghe SP, Sutanto MM, Danos AM, Johnson DN, Brady MJ, Cohen RN. Samarasinghe SP, et al. Obesity (Silver Spring). 2009 May;17(5):965-72. doi: 10.1038/oby.2008.629. Epub 2009 Jan 22. Obesity (Silver Spring). 2009. PMID: 19165156 Free PMC article. - Adipocyte differentiation of 3T3-L1 preadipocytes is dependent on lipoxygenase activity during the initial stages of the differentiation process.
Madsen L, Petersen RK, Sørensen MB, Jørgensen C, Hallenborg P, Pridal L, Fleckner J, Amri EZ, Krieg P, Furstenberger G, Berge RK, Kristiansen K. Madsen L, et al. Biochem J. 2003 Nov 1;375(Pt 3):539-49. doi: 10.1042/bj20030503. Biochem J. 2003. PMID: 18320708 - De-novo identification of PPARgamma/RXR binding sites and direct targets during adipogenesis.
Hamza MS, Pott S, Vega VB, Thomsen JS, Kandhadayar GS, Ng PW, Chiu KP, Pettersson S, Wei CL, Ruan Y, Liu ET. Hamza MS, et al. PLoS One. 2009;4(3):e4907. doi: 10.1371/journal.pone.0004907. Epub 2009 Mar 20. PLoS One. 2009. PMID: 19300518 Free PMC article. - The elusive endogenous adipogenic PPARγ agonists: Lining up the suspects.
Hallenborg P, Petersen RK, Kouskoumvekaki I, Newman JW, Madsen L, Kristiansen K. Hallenborg P, et al. Prog Lipid Res. 2016 Jan;61:149-62. doi: 10.1016/j.plipres.2015.11.002. Epub 2015 Dec 15. Prog Lipid Res. 2016. PMID: 26703188 Review. - [Characterization of novel genes regulating adipocyte differentiation].
Johmura Y. Johmura Y. Yakugaku Zasshi. 2007 Jan;127(1):135-42. doi: 10.1248/yakushi.127.135. Yakugaku Zasshi. 2007. PMID: 17202794 Review. Japanese.
Cited by
- Characteristic and fate determination of adipose precursors during adipose tissue remodeling.
Ye J, Gao C, Liang Y, Hou Z, Shi Y, Wang Y. Ye J, et al. Cell Regen. 2023 May 4;12(1):13. doi: 10.1186/s13619-023-00157-8. Cell Regen. 2023. PMID: 37138165 Free PMC article. Review. - Arachidonic Acid Added during the Differentiation Phase of 3T3-L1 Cells Exerts Anti-Adipogenic Effect by Reducing the Effects of Pro-Adipogenic Prostaglandins.
Nartey MNN, Jisaka M, Syeda PK, Nishimura K, Shimizu H, Yokota K. Nartey MNN, et al. Life (Basel). 2023 Jan 29;13(2):367. doi: 10.3390/life13020367. Life (Basel). 2023. PMID: 36836723 Free PMC article. - A simplified and defined serum-free medium for cultivating fat across species.
Mitić R, Cantoni F, Börlin CS, Post MJ, Jackisch L. Mitić R, et al. iScience. 2022 Dec 17;26(1):105822. doi: 10.1016/j.isci.2022.105822. eCollection 2023 Jan 20. iScience. 2022. PMID: 36636339 Free PMC article. - Bifidobacterium bifidum DS0908 and Bifidobacterium longum DS0950 Culture-Supernatants Ameliorate Obesity-Related Characteristics in Mice with High-Fat Diet-Induced Obesity.
Rahman MS, Lee Y, Park DS, Kim YS. Rahman MS, et al. J Microbiol Biotechnol. 2023 Jan 28;33(1):96-105. doi: 10.4014/jmb.2210.10046. Epub 2022 Nov 22. J Microbiol Biotechnol. 2023. PMID: 36457182 Free PMC article. - PPARγ phase separates with RXRα at PPREs to regulate target gene expression.
Li Z, Luo L, Yu W, Li P, Ou D, Liu J, Ma H, Sun Q, Liang A, Huang C, Chi T, Huang X, Zhang Y. Li Z, et al. Cell Discov. 2022 Apr 26;8(1):37. doi: 10.1038/s41421-022-00388-0. Cell Discov. 2022. PMID: 35473936 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources