Phospholipid metabolism regulated by a transcription factor sensing phosphatidic acid - PubMed (original) (raw)
. 2004 Jun 11;304(5677):1644-7.
doi: 10.1126/science.1096083.
Affiliations
- PMID: 15192221
- DOI: 10.1126/science.1096083
Phospholipid metabolism regulated by a transcription factor sensing phosphatidic acid
C J R Loewen et al. Science. 2004.
Abstract
Cells regulate the biophysical properties of their membranes by coordinated synthesis of different classes of lipids. Here, we identified a highly dynamic feedback mechanism by which the budding yeast Saccharomyces cerevisiae can regulate phospholipid biosynthesis. Phosphatidic acid on the endoplasmic reticulum directly bound to the soluble transcriptional repressor Opi1p to maintain it as inactive outside the nucleus. After the addition of the lipid precursor inositol, this phosphatidic acid was rapidly consumed, releasing Opi1p from the endoplasmic reticulum and allowing its nuclear translocation and repression of target genes. Thus, phosphatidic acid appears to be both an essential ubiquitous metabolic intermediate and a signaling lipid.
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