Engineered riboregulators enable post-transcriptional control of gene expression - PubMed (original) (raw)
doi: 10.1038/nbt986. Epub 2004 Jun 20.
Affiliations
- PMID: 15208640
- DOI: 10.1038/nbt986
Engineered riboregulators enable post-transcriptional control of gene expression
Farren J Isaacs et al. Nat Biotechnol. 2004 Jul.
Abstract
Recent studies have demonstrated the important enzymatic, structural and regulatory roles of RNA in the cell. Here we present a post-transcriptional regulation system in Escherichia coli that uses RNA to both silence and activate gene expression. We inserted a complementary cis sequence directly upstream of the ribosome binding site in a target gene. Upon transcription, this cis-repressive sequence causes a stem-loop structure to form at the 5'-untranslated region of the mRNA. The stem-loop structure interferes with ribosome binding, silencing gene expression. A small noncoding RNA that is expressed in trans targets the cis-repressed RNA with high specificity, causing an alteration in the stem-loop structure that activates expression. Such engineered riboregulators may lend insight into mechanistic actions of endogenous RNA-based processes and could serve as scalable components of biological networks, able to function with any promoter or gene to directly control gene expression.
Comment in
- Custom design of metabolism.
Liao JC. Liao JC. Nat Biotechnol. 2004 Jul;22(7):823-4. doi: 10.1038/nbt0704-823. Nat Biotechnol. 2004. PMID: 15229541 No abstract available.
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