Increased susceptibility of secretor factor gene Fut2-null mice to experimental vaginal candidiasis - PubMed (original) (raw)

Increased susceptibility of secretor factor gene Fut2-null mice to experimental vaginal candidiasis

Elizabeth A Hurd et al. Infect Immun. 2004 Jul.

Abstract

Fut2-LacZ-null mice, which are a model of the human ABO and Lewis nonsecretor group, display increased susceptibility to experimental yeast vaginitis, indicating a role for alpha(1,2)fucosylated cervical glycans in mucosal defense. However, the lack of significant effect of competitive inhibition by exogenous neoglycoproteins in this study emphasizes the complexity of Candida-epithelial cell adhesion events.

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Figures

FIG. 1.

FIG. 1.

Cell-specific expression of Fut2-LacZ reporter gene activity in endocervical glandular cells and loss of α(1,2)fucosylated glycans from the lower reproductive tract of Fut2-LacZ-null mice. Fut2-LacZ-null and C57BL/6J wild-type mice were processed for X-Gal staining (top panels) and UEA-I lectin staining (bottom panels). Specific nuclear X-Gal staining was detected in endocervical glandular epithelium of Fut2-LacZ-null mice in estrus (A), while staining in wild-type mice was negative (B). UEA-I lectin staining was absent from Fut2-LacZ-null mice (C) but was associated with endocervical and upper vaginal epithelia of wild-type mice (D), indicating that Fut2 is essential for expression of lower reproductive tract α(1,2)fucosylated glycans. Endocervical (EC) and vaginal (V) epithelium are marked. The asterisk indicates the location of the squamocolumnar epithelial junction of the cervix. Bar, 100 μm.

FIG. 2.

FIG. 2.

Quantification of experimental C. albicans vaginitis in three mouse genotypes. Fut2-LacZ-null mice, Fut1-null mice, and wild-type mice were subjected to C. albicans vaginitis. The experiment was performed in triplicate with similar results; cumulative data are shown (n = 7 to 8 mice per group). One-way ANOVA and post-hoc analysis revealed that Fut2-LacZ-null mice display an increased susceptibility (**, P < 0.01) versus wild-type mice at 4 and 7 days postinoculation. Error bars show the standard errors of the means.

FIG. 3.

FIG. 3.

Effects of neoglycoproteins on susceptibility of Fut2-LacZ-null and wild-type mice to experimental yeast vaginitis. C57BL/6J wild-type (A) and Fut2-LacZ-null (B) mice were inoculated with C. albicans cells preincubated with BSA, Fuc-BSA, or Gal-BSA (n = 9 to 11 mice per group). Error bars show the standard errors of the means. Under the conditions tested, exogenous neoglycoproteins were not effective in preventing C. albicans colonization in Fut2-LacZ-null or wild-type mice.

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