A defined human system that supports bidirectional mismatch-provoked excision - PubMed (original) (raw)
A defined human system that supports bidirectional mismatch-provoked excision
Leonid Dzantiev et al. Mol Cell. 2004.
Free article
Abstract
Mismatch-provoked excision directed by a strand break located 3' or 5' to the mispair has been reconstituted using purified human proteins. While MutSalpha, EXOI, and RPA are sufficient to support hydrolysis directed by a 5' strand break, 3' directed excision also requires MutLalpha, PCNA, and RFC. EXOI interacts with PCNA. RFC and PCNA suppress EXOI-mediated 5' to 3' hydrolysis when the nick that directs excision is located 3' to the mispair and activate 3' to 5' excision, which is dependent on loaded PCNA and apparently mediated by a cryptic EXOI 3' to 5' hydrolytic function. By contrast, RFC and PCNA have only a limited effect on 5' to 3' excision directed by a 5' strand break.
Comment in
- Replication factors license exonuclease I in mismatch repair.
Surtees JA, Alani E. Surtees JA, et al. Mol Cell. 2004 Jul 23;15(2):164-6. doi: 10.1016/j.molcel.2004.07.004. Mol Cell. 2004. PMID: 15260965
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