Analysis of the set of GABA(A) receptor genes in the human genome - PubMed (original) (raw)
. 2004 Oct 1;279(40):41422-35.
doi: 10.1074/jbc.M401354200. Epub 2004 Jul 16.
Affiliations
- PMID: 15258161
- DOI: 10.1074/jbc.M401354200
Free article
Analysis of the set of GABA(A) receptor genes in the human genome
Joseph Simon et al. J Biol Chem. 2004.
Free article
Abstract
The genes of the ionotropic gamma-aminobutyric acid receptor (GABR) subunits have shown an unusual chromosomal clustering, but only now can this be fully specified by analyses of the human genome. We have characterized the genes encoding the 18 known human GABR subunits, plus one now located here, for their precise locations, sizes, and exon/intron structures. Clusters of 17 of the 19, distributed between five chromosomes, are specified in detail, and their possible significance is considered. By applying search algorithms designed to recognize sequences of all known GABR-type subunits in species from man down to nematodes, we found no new GABR subunit is detectable in the human genome. However, the sequence of the human orthologue of the rat GABR rho3 receptor subunit was uncovered by these algorithms, and its gene could be analyzed. Consistent with those search results, orthologues of the beta4 and gamma4 subunits from the chicken, not cloned from mammals, were not detectable in the human genome by specific searches for them. The relationships are consistent with the mammalian subunit being derived from the beta line and epsilon from the gamma line, with mammalian loss of beta4 and gamma4. In their structures the human GABR genes show a basic pattern of nine coding exons, with six different genomic mechanisms for the alternative splicing found in various subunits. Additional noncoding exons occur for certain subunits, which can be regulatory. A dicysteine loop and its exon show remarkable constancy between all GABR subunits and species, of deduced functional significance.
Similar articles
- Consequences of the evolution of the GABA(A) receptor gene family.
Darlison MG, Pahal I, Thode C. Darlison MG, et al. Cell Mol Neurobiol. 2005 Jun;25(3-4):607-24. doi: 10.1007/s10571-005-4004-4. Cell Mol Neurobiol. 2005. PMID: 16075381 Review. - The evolution of GABAA receptor-like genes.
Tsang SY, Ng SK, Xu Z, Xue H. Tsang SY, et al. Mol Biol Evol. 2007 Feb;24(2):599-610. doi: 10.1093/molbev/msl188. Epub 2006 Nov 29. Mol Biol Evol. 2007. PMID: 17135332 - A sequence-ready BAC contig of the GABAA receptor gene cluster Gabrg1-Gabra2-Gabrb1 on mouse chromosome 5.
Lengeling A, Wiltshire T, Otmani C, Bucán M. Lengeling A, et al. Genome Res. 1999 Aug;9(8):732-8. Genome Res. 1999. PMID: 10447508 Free PMC article. - Human chromosomal localization of genes encoding the gamma 1 and gamma 2 subunits of the gamma-aminobutyric acid receptor indicates that members of this gene family are often clustered in the genome.
Wilcox AS, Warrington JA, Gardiner K, Berger R, Whiting P, Altherr MR, Wasmuth JJ, Patterson D, Sikela JM. Wilcox AS, et al. Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5857-61. doi: 10.1073/pnas.89.13.5857. Proc Natl Acad Sci U S A. 1992. PMID: 1321425 Free PMC article. - GABAA receptors: building the bridge between subunit mRNAs, their promoters, and cognate transcription factors.
Steiger JL, Russek SJ. Steiger JL, et al. Pharmacol Ther. 2004 Mar;101(3):259-81. doi: 10.1016/j.pharmthera.2003.12.002. Pharmacol Ther. 2004. PMID: 15031002 Review.
Cited by
- Modular Structure and Polymerization Status of GABAA Receptors Illustrated with EM Analysis and AlphaFold2 Prediction.
Kan C, Ullah A, Dang S, Xue H. Kan C, et al. Int J Mol Sci. 2024 Sep 21;25(18):10142. doi: 10.3390/ijms251810142. Int J Mol Sci. 2024. PMID: 39337627 Free PMC article. - A bupropion modulatory site in the Gloeobacter violaceus ligand-gated ion channel.
Do HQ, Pirayesh E, Ferreira G, Pandhare A, Gallardo ZR, Jansen M. Do HQ, et al. Biophys J. 2024 Jul 16;123(14):2185-2198. doi: 10.1016/j.bpj.2024.04.027. Epub 2024 Apr 27. Biophys J. 2024. PMID: 38678367 Free PMC article. - Gene Expression of GABAA Receptor Subunits and Association with Patient Survival in Glioma.
Badalotti R, Dalmolin M, Malafaia O, Ribas Filho JM, Roesler R, Fernandes MAC, Isolan GR. Badalotti R, et al. Brain Sci. 2024 Mar 14;14(3):275. doi: 10.3390/brainsci14030275. Brain Sci. 2024. PMID: 38539663 Free PMC article. - Alfaxalone does not have long-term effects on goldfish pyramidal neuron action potential properties or GABAA receptor currents.
Di Stefano D, Suganthan H, Buck L. Di Stefano D, et al. FEBS Open Bio. 2024 Apr;14(4):555-573. doi: 10.1002/2211-5463.13777. Epub 2024 Feb 11. FEBS Open Bio. 2024. PMID: 38342633 Free PMC article. - The effect of psychoactive bacteria, Bifidobacterium longum Rosell®-175 and Lactobacillus rhamnosus JB-1, on brain proteome profiles in mice.
Jarosz ŁS, Socała K, Michalak K, Wiater A, Ciszewski A, Majewska M, Marek A, Grądzki Z, Wlaź P. Jarosz ŁS, et al. Psychopharmacology (Berl). 2024 May;241(5):925-945. doi: 10.1007/s00213-023-06519-z. Epub 2023 Dec 29. Psychopharmacology (Berl). 2024. PMID: 38156998 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources