Nitric oxide as an endogenous mutagen for Sendai virus without antiviral activity - PubMed (original) (raw)

Mutation frequency (A and C) and virus yield (B and D) in iNOS-SW480 cells and parent SW480 cells with or without

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-NMMA and

d

-NMMA. The mutagenic potential of NO for SeV was determined by analysis of the mutation of GFP-SeV. (A and B) Monolayers of iNOS-SW480 cells were inoculated with GFP-SeV at a multiplicity of infection of 3.0 PFU per cell, followed by culture with or without

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-NMMA or

d

-NMMA. The culture supernatant obtained 48 h after infection was used for determination of viral mutation by the mutation assay (A). The virus yield in the same samples used for the mutation assay is shown in B. (C and D) Monolayers of iNOS-SW480 cells and SW480 cells were inoculated with GFP-SeV at a multiplicity of infection of 0.1 PFU per cell (multicycle replications). After 72 h of culture of the infected cells with or without

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-NMMA, the mutation frequency (C) and virus yield (D) were assessed in the same manner as in A and B. Control, iNOS-SW480 cells without

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-NMMA;

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-NMMA (1.0 or 10 mM), iNOS-SW480 cells treated with 1.0 or 10 mM

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-NMMA;

d

-NMMA, iNOS-SW480 cells treated with 10 mM

d

-NMMA. Data are means ± standard error of four independent experiments (>1,000 plaques counted/assay); *, P < 0.05, and **, P < 0.01 versus the control and the

d

-NMMA-treated cells. See text for details.