Crystallization of macromolecules in silica gels - PubMed (original) (raw)
Crystallization of macromolecules in silica gels
R Cudney et al. Acta Crystallogr D Biol Crystallogr. 1994.
Abstract
Procedures are described for the crystallization of proteins, nucleic acids and viruses in a silica-gel matrix using otherwise standard reagents and conditions. Methods are given based on both vapor diffusion in a sitting drop and liquid-liquid diffusion. Using a variety of macromolecules our results suggest that the gel matrix suppresses nucleation, reduces the rate of growth, and generally leads to larger, higher-quality crystals of enhanced stability. Presumably these effects arise from the decreased mobility of the macromolecules and their flux at the crystal surface during growth.
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