RhoH is required to maintain the integrin LFA-1 in a nonadhesive state on lymphocytes - PubMed (original) (raw)

doi: 10.1038/ni1103. Epub 2004 Aug 8.

Affiliations

PMID: 15300248
DOI: 10.1038/ni1103

RhoH is required to maintain the integrin LFA-1 in a nonadhesive state on lymphocytes

Lisa K Cherry et al. Nat Immunol. 2004 Sep.

Abstract

Lymphocyte function-associated antigen 1 (LFA-1) is relatively nonadhesive on resting lymphocytes; however, the mechanisms underlying changes in its adhesiveness are poorly understood. In this study, we generated a Jurkat T cell clone, J+hi1.14, that contained low amounts of mRNA for RhoH, a leukocyte-specific inhibitory Rho family member. J+hi1.14 cells expressed constitutively adhesive LFA-1 and the cells bound spontaneously to intracellular adhesion molecules 1, 2 and 3. Reconstitution of RhoH mRNA expression in J+hi1.14 cells reverted the adhesion phenotype to that of wild-type. We obtained similar results using RNA interference in peripheral blood lymphocytes. These data demonstrate that RhoH is required for maintenance of lymphocyte LFA-1 in a nonadhesive state.

PubMed Disclaimer

Cited by

A Novel Homozygous RHOH Variant Associated with T Cell Dysfunction and Recurrent Opportunistic Infections.
Zhou J, Qian M, Jiang N, Wu J, Feng X, Yu M, Min Q, Xu H, Yang Y, Yang Q, Zhou F, Shao L, Zhu H, Yang Y, Wang JY, Ruan Q, Zhang W. Zhou J, et al. J Clin Immunol. 2024 May 22;44(6):131. doi: 10.1007/s10875-024-01735-4. J Clin Immunol. 2024. PMID: 38775840
ISG20L2: an RNA nuclease regulating T cell activation.
Rodríguez-Galán A, Dosil SG, Hrčková A, Fernández-Messina L, Feketová Z, Pokorná J, Fernández-Delgado I, Camafeita E, Gómez MJ, Ramírez-Huesca M, Gutiérrez-Vázquez C, Sánchez-Cabo F, Vázquez J, Vaňáčová Š, Sánchez-Madrid F. Rodríguez-Galán A, et al. Cell Mol Life Sci. 2023 Aug 30;80(9):273. doi: 10.1007/s00018-023-04925-2. Cell Mol Life Sci. 2023. PMID: 37646974 Free PMC article.
Nanoengineering facilitating the target mission: targeted extracellular vesicles delivery systems design.
Song H, Chen X, Hao Y, Wang J, Xie Q, Wang X. Song H, et al. J Nanobiotechnology. 2022 Sep 29;20(1):431. doi: 10.1186/s12951-022-01638-9. J Nanobiotechnology. 2022. PMID: 36175866 Free PMC article. Review.
Bimodal expression of RHOH during myelomonocytic differentiation: Implications for the expansion of AML differentiation therapy.
Galiègue-Zouitina S, Fu Q, Carton-Latreche C, Poret N, Cheok M, Leprêtre F, Figeac M, Quesnel B, El Bouazzati H, Shelley CS. Galiègue-Zouitina S, et al. EJHaem. 2021 Jan 20;2(2):196-210. doi: 10.1002/jha2.128. eCollection 2021 May. EJHaem. 2021. PMID: 35845268 Free PMC article.
PI3K in T Cell Adhesion and Trafficking.
Johansen KH, Golec DP, Thomsen JH, Schwartzberg PL, Okkenhaug K. Johansen KH, et al. Front Immunol. 2021 Aug 6;12:708908. doi: 10.3389/fimmu.2021.708908. eCollection 2021. Front Immunol. 2021. PMID: 34421914 Free PMC article. Review.

RhoH is required to maintain the integrin LFA-1 in a nonadhesive state on lymphocytes - PubMed (original) (raw)