Expression of TREM-1 mRNA in acute pancreatitis - PubMed (original) (raw)

Expression of TREM-1 mRNA in acute pancreatitis

Da-Yu Wang et al. World J Gastroenterol. 2004.

Abstract

Aim: To explore the expression of triggering receptor expressed on myeloid cells (TREM-1) mRNA in acute pancreatitis (AP).

Methods: Using the reverse transcription polymerase chain reaction (RT-PCR), we examined the expression of TREM-1 mRNA in 10 cases of mild acute pancreatitis (MAP), 8 cases of severe acute pancreatitis (SAP), and 10 cases of healthy control subjects. And we also examined the expression of TREM-1 mRNA in 14 cases of AP (including 10 MAP and 4 SAP) before treatment, after successful therapy and clinically cured.

Results: The expression of TREM-1 mRNA in the groups of MAP, SAP patients and healthy control subjects was 0.771+/-0.274, 1.092+/-0.331 and 0.459+/-0.175, respectively; there was a significant difference among the three groups (P<0.05). And there was also a significant difference between the AP patients (0.914+/-0.341) and healthy control subjects (0.459+/-0.175) (P<0.05). Moreover, in the 14 cases of AP, before treatment, after successful therapy and clinically cured, the expression of TREM-1 mRNA was 0.905+/-0.226, 0.739+/-0.169 and 0.633+/-0.140, respectively, and there was a significant difference among the three stages (P<0.05).

Conclusion: The expression of TREM-1 mRNA in the patients with AP increases obviously, and correlates with the degree of AP. Furthermore, the expression of TREM-1 mRNA is distinctly different at the different stages of AP. It indicates TREM-1 may play an important role in the occurrence and development of AP.

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Figures

Figure 1

Figure 1

Electrophoresis of the PCR products. M: DL2000 marker; 1: the PCR product of normal subject; 2: the PCR product of MAP; 3: the PCR product of SAP. The amplified fragment lengths of β-actin and TREM-1 are 520 bp and 232 bp respectively.

Figure 2

Figure 2

The A ratios of TREM-1/β-actin in different degree of AP: There was a significant difference among the three groups in the expressions of TREM-1 mRNA (P < 0.05). And between the AP patients and healthy control subjects, the difference was significant (P < 0.05).

Figure 3

Figure 3

Electrophoresis of the PCR products. M: DL2000 marker; 1: the AP patient before treatment; 2: the same patient after successful therapy; 3: the same AP patient after clinically cured; 4: negative control. The amplified fragment length of β-actin is 520 bp, of TREM-1 is 232 bp. 10 g/L agarose gel.

Figure 4

Figure 4

The A ratios of TREM-1/β-actin in different stage of AP: 1, the group in the stage of before treatment. 2, the group in the stage after successful therapy. 3, the group in the stage of clinically cured. 4, the group of normal subject. There was a significant difference among the three stages in the expression of TREM-1 mRNA (groups 1, 2 and 3. P < 0.05). And in the group of clinically cured the ratio was higher than in healthy control subjects (P < 0.05).

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