Cryo-EM visualization of a viral internal ribosome entry site bound to human ribosomes: the IRES functions as an RNA-based translation factor - PubMed (original) (raw)
. 2004 Aug 20;118(4):465-75.
doi: 10.1016/j.cell.2004.08.001.
Affiliations
- PMID: 15315759
- DOI: 10.1016/j.cell.2004.08.001
Free article
Cryo-EM visualization of a viral internal ribosome entry site bound to human ribosomes: the IRES functions as an RNA-based translation factor
Christian M T Spahn et al. Cell. 2004.
Free article
Abstract
Internal initiation of protein synthesis in eukaryotes is accomplished by recruitment of ribosomes to structured internal ribosome entry sites (IRESs), which are located in certain viral and cellular messenger RNAs. An IRES element in cricket paralysis virus (CrPV) can directly assemble 80S ribosomes in the absence of canonical initiation factors and initiator tRNA. Here we present cryo-EM structures of the CrPV IRES bound to the human ribosomal 40S subunit and to the 80S ribosome. The CrPV IRES adopts a defined, elongate structure within the ribosomal intersubunit space and forms specific contacts with components of the ribosomal A, P, and E sites. Conformational changes in the ribosome as well as within the IRES itself show that CrPV IRES actively manipulates the ribosome. CrPV-like IRES elements seem to act as RNA-based translation factors.
Similar articles
- Structure of the ribosome-bound cricket paralysis virus IRES RNA.
Schüler M, Connell SR, Lescoute A, Giesebrecht J, Dabrowski M, Schroeer B, Mielke T, Penczek PA, Westhof E, Spahn CM. Schüler M, et al. Nat Struct Mol Biol. 2006 Dec;13(12):1092-6. doi: 10.1038/nsmb1177. Epub 2006 Nov 19. Nat Struct Mol Biol. 2006. PMID: 17115051 - Coordinated assembly of human translation initiation complexes by the hepatitis C virus internal ribosome entry site RNA.
Ji H, Fraser CS, Yu Y, Leary J, Doudna JA. Ji H, et al. Proc Natl Acad Sci U S A. 2004 Dec 7;101(49):16990-5. doi: 10.1073/pnas.0407402101. Epub 2004 Nov 24. Proc Natl Acad Sci U S A. 2004. PMID: 15563596 Free PMC article. - Translation initiation by factor-independent binding of eukaryotic ribosomes to internal ribosomal entry sites.
Pisarev AV, Shirokikh NE, Hellen CU. Pisarev AV, et al. C R Biol. 2005 Jul;328(7):589-605. doi: 10.1016/j.crvi.2005.02.004. C R Biol. 2005. PMID: 15992743 Review. - Hepatitis C virus IRES RNA-induced changes in the conformation of the 40s ribosomal subunit.
Spahn CM, Kieft JS, Grassucci RA, Penczek PA, Zhou K, Doudna JA, Frank J. Spahn CM, et al. Science. 2001 Mar 9;291(5510):1959-62. doi: 10.1126/science.1058409. Science. 2001. PMID: 11239155 - Structural and mechanistic insights into hepatitis C viral translation initiation.
Fraser CS, Doudna JA. Fraser CS, et al. Nat Rev Microbiol. 2007 Jan;5(1):29-38. doi: 10.1038/nrmicro1558. Epub 2006 Nov 27. Nat Rev Microbiol. 2007. PMID: 17128284 Review.
Cited by
- GWIPS-viz as a tool for exploring ribosome profiling evidence supporting the synthesis of alternative proteoforms.
Michel AM, Ahern AM, Donohue CA, Baranov PV. Michel AM, et al. Proteomics. 2015 Jul;15(14):2410-6. doi: 10.1002/pmic.201400603. Epub 2015 Apr 23. Proteomics. 2015. PMID: 25736862 Free PMC article. - Kinetics of initiating polypeptide elongation in an IRES-dependent system.
Zhang H, Ng MY, Chen Y, Cooperman BS. Zhang H, et al. Elife. 2016 Jun 2;5:e13429. doi: 10.7554/eLife.13429. Elife. 2016. PMID: 27253065 Free PMC article. - One core, two shells: bacterial and eukaryotic ribosomes.
Melnikov S, Ben-Shem A, Garreau de Loubresse N, Jenner L, Yusupova G, Yusupov M. Melnikov S, et al. Nat Struct Mol Biol. 2012 Jun 5;19(6):560-7. doi: 10.1038/nsmb.2313. Nat Struct Mol Biol. 2012. PMID: 22664983 Review. - Conservation and diversity among the three-dimensional folds of the Dicistroviridae intergenic region IRESes.
Pfingsten JS, Costantino DA, Kieft JS. Pfingsten JS, et al. J Mol Biol. 2007 Jul 27;370(5):856-69. doi: 10.1016/j.jmb.2007.04.076. Epub 2007 May 8. J Mol Biol. 2007. PMID: 17544444 Free PMC article. - Mechanism of translation initiation by Dicistroviridae IGR IRESs.
Hertz MI, Thompson SR. Hertz MI, et al. Virology. 2011 Mar 15;411(2):355-61. doi: 10.1016/j.virol.2011.01.005. Epub 2011 Feb 1. Virology. 2011. PMID: 21284991 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources