Nonspecific proliferative responses of murine lymphocytes to Borrelia burgdorferi antigens - PubMed (original) (raw)
Nonspecific proliferative responses of murine lymphocytes to Borrelia burgdorferi antigens
M S de Souza et al. J Infect Dis. 1992 Mar.
Abstract
Proliferative responses of naive splenocytes to Borrelia burgdorferi antigens from mice susceptible (C3H) and resistant (BALB) to Lyme borreliosis were investigated. B. burgdorferi spirochetes and recombinant outer surface proteins, OspA and OspB, were found to induce nonspecific proliferation of naive splenocytes from both strains of mice. Cell purification studies localized nonspecific proliferation to the B cell-enriched fraction. B. burgdorferi, OspA, and OspB were found to induce IgM and IgG synthesis in vitro. The mitogenic effect of B. burgdorferi was dissimilar to that of lipopolysaccharide (LPS), in that B cells from C3H/HeJ mice (LPS-unresponsive) responded at levels comparable to those from C3H/HeNCrlBr mice. These results emphasize the need for caution in the study of antigen-specific proliferation for B. burgdorferi.
Comment in
- Lymphocyte proliferation assay in Lyme disease.
Wallach FR, Murray HW. Wallach FR, et al. J Infect Dis. 1992 Oct;166(4):938-9. doi: 10.1093/infdis/166.4.938-a. J Infect Dis. 1992. PMID: 1527433 No abstract available.
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