Local genetic patterns within a vancomycin-resistant Enterococcus faecalis clone isolated in three hospitals in Portugal - PubMed (original) (raw)

Local genetic patterns within a vancomycin-resistant Enterococcus faecalis clone isolated in three hospitals in Portugal

Carla Novais et al. Antimicrob Agents Chemother. 2004 Sep.

Abstract

Eight pulsed-field gel electrophoresis subtypes and six Tn1546 variants were identified among Enterococcus faecalis isolates of a single clone recovered in three geographically separate Portuguese hospitals. Some clonal subtypes were found in particular hospitals, and Tn1546 variants were either widespread or confined to some of them. We also report on the first Tn1546 transposon containing an ISEf1 insertion.

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Figures

FIG. 1.

FIG. 1.

SmaI-digested chromosomal DNA of vancomycin-susceptible and vancomycin-resistant E. faecalis isolates classified as clonal type B, as determined by PFGE. Lanes 1, 3, 4, 5, and 6, subtype B; lanes 7 and 12, subtype B1; lane 11, subtype B1′; lanes 9 and 10, subtype B2; lane 8, subtype B3; lane 13, subtype B3′; lane 14, subtype B4; lanes 15 and 16, subtype B5.

References

    1. Bonten, M. J. M., R. J. Willems, and R. A. Weinstein. 2001. Vancomycin resistant enterococci: why are they here, and where do they come from? Lancet Infect. Dis. 1**:**314-325. -PubMed
    1. Borgen, K., M. Sorum, Y. Wasteson, H. Kruse, and H. Oppegaard. 2002. Genetic linkage between erm(B) and vanA in Enterococcus hirae of poultry origin. Microb. Drug Resist. 8**:**363-368. -PubMed
    1. Dahl, K. H., and A. Sundsfjord. 2003. Transferable vanB2 Tn_5382_-containing elements in fecal streptococcal strains from veal calves. Antimicrob. Agents Chemother. 47**:**2579-2583. -PMC -PubMed
    1. Darini, A. L. C., M. F. I. Palepou, and N. Woodford. 1999. Nucleotide sequence of IS_1542_, an insertion sequence identified within VanA glycopeptide resistance elements of enterococci. FEMS Microbiol. Lett. 173**:**341-346. -PubMed
    1. De Lencastre, H., A. E. Brown, M. Chung, D. Armstrong, and A. Tomasz. 1999. Role of the transposon Tn_5482_ in the epidemiology of vancomycin resistant Enterococcus faecium in the pediatric oncology unit of a New York City Hospital. Microb. Drug Resist. 5**:**113-129. -PubMed

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