Differentiation of mouse embryonic stem cells into a defined neuronal lineage - PubMed (original) (raw)
Comparative Study
doi: 10.1038/nn1301.
Affiliations
- PMID: 15332090
- DOI: 10.1038/nn1301
Comparative Study
Differentiation of mouse embryonic stem cells into a defined neuronal lineage
Miriam Bibel et al. Nat Neurosci. 2004 Sep.
Abstract
Although it has long been known that cultured embryonic stem cells can generate neurons, the lineage relationships with their immediate precursors remain unclear. We report here that selection of highly proliferative stem cells followed by treatment with retinoic acid generated essentially pure precursors that markers identified as Pax-6-positive radial glial cells. As they do in vivo, these cells went on to generate neurons with remarkably uniform biochemical and electrophysiological characteristics.
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