Secretion of T cell receptor fragments from recombinant Escherichia coli cells - PubMed (original) (raw)
Secretion of T cell receptor fragments from recombinant Escherichia coli cells
E S Ward. J Mol Biol. 1992.
Abstract
This study describes the secretion and purification of T cell receptor (TCR) V alpha, V beta domains and single chain V alpha-V beta fragments (scTCRs) from recombinant Escherichia coli cells. The TCR V alpha and V beta genes are derived from a T cell hybridoma that is associated with disease pathogenesis in murine experimental allergic encephalomyelitis (EAE). Circular dichroism (c.d.) analyses of the single domains and the scTCR indicate that they are folded into beta-pleated sheet structures similar to those of immunoglobulin variable domains. The secreted TCR fragments can be purified in milligram quantities, and could therefore be used in high-resolution structural studies, in immunization to generate anti-clonotypic antibodies or in vaccination.
Similar articles
- Expression and secretion of T-cell receptor V alpha and V beta domains using Escherichia coli as a host.
Ward ES. Ward ES. Scand J Immunol. 1991 Aug;34(2):215-20. doi: 10.1111/j.1365-3083.1991.tb01539.x. Scand J Immunol. 1991. PMID: 1830971 - A T cell receptor V alpha domain expressed in bacteria: does it dimerize in solution?
Plaksin D, Chacko S, McPhie P, Bax A, Padlan EA, Margulies DH. Plaksin D, et al. J Exp Med. 1996 Oct 1;184(4):1251-8. doi: 10.1084/jem.184.4.1251. J Exp Med. 1996. PMID: 8879196 Free PMC article. - The V-region disease hypothesis: new evidence suggests it is probably wrong.
Wilson DB, Steinman L, Gold DP. Wilson DB, et al. Immunol Today. 1993 Aug;14(8):376-80; discussion 380-2. doi: 10.1016/0167-5699(93)90136-9. Immunol Today. 1993. PMID: 7691066 Review.
Cited by
- Engineering a HER2-specific antibody-drug conjugate to increase lysosomal delivery and therapeutic efficacy.
Kang JC, Sun W, Khare P, Karimi M, Wang X, Shen Y, Ober RJ, Ward ES. Kang JC, et al. Nat Biotechnol. 2019 May;37(5):523-526. doi: 10.1038/s41587-019-0073-7. Epub 2019 Apr 1. Nat Biotechnol. 2019. PMID: 30936563 Free PMC article. - Direct expression of active human tissue inhibitors of metalloproteinases by periplasmic secretion in Escherichia coli.
Lee KB, Nam DH, Nuhn JAM, Wang J, Schneider IC, Ge X. Lee KB, et al. Microb Cell Fact. 2017 Apr 28;16(1):73. doi: 10.1186/s12934-017-0686-9. Microb Cell Fact. 2017. PMID: 28454584 Free PMC article. - Loss of expression of the recycling receptor, FcRn, promotes tumor cell growth by increasing albumin consumption.
Swiercz R, Mo M, Khare P, Schneider Z, Ober RJ, Ward ES. Swiercz R, et al. Oncotarget. 2017 Jan 10;8(2):3528-3541. doi: 10.18632/oncotarget.13869. Oncotarget. 2017. PMID: 27974681 Free PMC article. - Production of a soluble disulfide bond-linked TCR in the cytoplasm of Escherichia coli trxB gor mutants.
Liddy N, Molloy PE, Bennett AD, Boulter JM, Jakobsen BK, Li Y. Liddy N, et al. Mol Biotechnol. 2010 Jun;45(2):140-9. doi: 10.1007/s12033-010-9250-0. Mol Biotechnol. 2010. PMID: 20143183 - Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA.
Gunnarsen KS, Lunde E, Kristiansen PE, Bogen B, Sandlie I, Løset GÅ. Gunnarsen KS, et al. BMC Biotechnol. 2010 Feb 3;10:8. doi: 10.1186/1472-6750-10-8. BMC Biotechnol. 2010. PMID: 20128915 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources