Subcellular sites for bacterial protein export - PubMed (original) (raw)
. 2004 Sep;53(6):1583-99.
doi: 10.1111/j.1365-2958.2004.04278.x.
Harold Tjalsma, Girbe Buist, Dariusz Stepniak, Michel Meijer, Marten Veenhuis, Martin Westermann, Jörg P Müller, Sierd Bron, Jan Kok, Oscar P Kuipers, Jan D H Jongbloed
Affiliations
- PMID: 15341641
- DOI: 10.1111/j.1365-2958.2004.04278.x
Free article
Subcellular sites for bacterial protein export
Nathalie Campo et al. Mol Microbiol. 2004 Sep.
Free article
Abstract
Most bacterial proteins destined to leave the cytoplasm are exported to extracellular compartments or imported into the cytoplasmic membrane via the highly conserved SecA-YEG pathway. In the present studies, the subcellular distributions of core components of this pathway, SecA and SecY, and of the secretory protein pre-AmyQ, were analysed using green fluorescent protein fusions, immunostaining and/or immunogold labelling techniques. It is shown that SecA, SecY and (pre-)AmyQ are located at specific sites near and/or in the cytoplasmic membrane of Bacillus subtilis. The localization patterns of these proteins suggest that the Sec machinery is organized in spiral-like structures along the cell, with most of the translocases organized in specific clusters along these structures. However, this localization appears to be independent of the helicoidal structures formed by the actin-like cytoskeletal proteins, MreB or Mbl. Interestingly, the specific localization of SecA is dynamic, and depends on active translation. Moreover, reducing the phosphatidylglycerol phospholipids content in the bacterial membrane results in delocalization of SecA, suggesting the involvement of membrane phospholipids in the localization process. These data show for the first time that, in contrast to the recently reported uni-ExPortal site in the coccoïd Streptococcus pyogenes, multiple sites dedicated to protein export are present in the cytoplasmic membrane of rod-shaped B. subtilis.
Comment in
- Traffic spotting: poles apart.
Pugsley AP, Buddelmeijer N. Pugsley AP, et al. Mol Microbiol. 2004 Sep;53(6):1559-62. doi: 10.1111/j.1365-2958.2004.04273.x. Mol Microbiol. 2004. PMID: 15341638
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