Human telomeres are attached to the nuclear matrix - PubMed (original) (raw)

Human telomeres are attached to the nuclear matrix

T de Lange. EMBO J. 1992 Feb.

Abstract

This report shows that human telomeres are tightly associated with the nuclear matrix. Telomere attachment is observed in several cell types and in all stages of interphase. Mapping experiments show that telomeres are anchored via their TTAGGG repeats; a subtelomeric repeat located immediately proximal to the telomeric TTAGGG repeats is quantitatively released from the nuclear matrix by restriction endonuclease cleavage. TTAGGG repeats introduced at chromosome-internal sites by DNA transfection do not behave as matrix attached loci, suggesting that the telomeric position of the repeats is required for their interaction with the nuclear matrix. These findings are consistent with the idea that telomeres function as a nucleoprotein complex.

PubMed Disclaimer

Similar articles

• Structure, subnuclear distribution, and nuclear matrix association of the mammalian telomeric complex.
  Ludérus ME, van Steensel B, Chong L, Sibon OC, Cremers FF, de Lange T. Ludérus ME, et al. J Cell Biol. 1996 Nov;135(4):867-81. doi: 10.1083/jcb.135.4.867. J Cell Biol. 1996. PMID: 8922373 Free PMC article.
• A novel telomere structure in a human alternative lengthening of telomeres cell line.
  Marciniak RA, Cavazos D, Montellano R, Chen Q, Guarente L, Johnson FB. Marciniak RA, et al. Cancer Res. 2005 Apr 1;65(7):2730-7. doi: 10.1158/0008-5472.CAN-04-2888. Cancer Res. 2005. PMID: 15805272
• A negative regulator of telomere-length protein trf1 is associated with interstitial (TTAGGG)n blocks in immortal Chinese hamster ovary cells.
  Krutilina RI, Oei S, Buchlow G, Yau PM, Zalensky AO, Zalenskaya IA, Bradbury EM, Tomilin NV. Krutilina RI, et al. Biochem Biophys Res Commun. 2001 Jan 19;280(2):471-5. doi: 10.1006/bbrc.2000.4143. Biochem Biophys Res Commun. 2001. PMID: 11162541
• Structure and variability of human chromosome ends.
  de Lange T, Shiue L, Myers RM, Cox DR, Naylor SL, Killery AM, Varmus HE. de Lange T, et al. Mol Cell Biol. 1990 Feb;10(2):518-27. doi: 10.1128/mcb.10.2.518-527.1990. Mol Cell Biol. 1990. PMID: 2300052 Free PMC article.
• TRF1, a mammalian telomeric protein.
  Smith S, de Lange T. Smith S, et al. Trends Genet. 1997 Jan;13(1):21-6. doi: 10.1016/s0168-9525(96)10052-4. Trends Genet. 1997. PMID: 9009844 Review.

Cited by

• Innovative Tools for DNA Topology Probing in Human Cells Reveal a Build-Up of Positive Supercoils Following Replication Stress at Telomeres and at the FRA3B Fragile Site.
  Ghilain C, Vidal-Cruchez O, Joly A, Debatisse M, Gilson E, Giraud-Panis MJ. Ghilain C, et al. Cells. 2024 Aug 15;13(16):1361. doi: 10.3390/cells13161361. Cells. 2024. PMID: 39195250 Free PMC article.
• Close Ties between the Nuclear Envelope and Mammalian Telomeres: Give Me Shelter.
  Pennarun G, Picotto J, Bertrand P. Pennarun G, et al. Genes (Basel). 2023 Mar 23;14(4):775. doi: 10.3390/genes14040775. Genes (Basel). 2023. PMID: 37107534 Free PMC article. Review.
• Ultrastructure and nuclear architecture of telomeric chromatin revealed by correlative light and electron microscopy.
  Hübner B, von Otter E, Ahsan B, Wee ML, Henriksson S, Ludwig A, Sandin S. Hübner B, et al. Nucleic Acids Res. 2022 May 20;50(9):5047-5063. doi: 10.1093/nar/gkac309. Nucleic Acids Res. 2022. PMID: 35489064 Free PMC article.
• Dynamics of nuclear matrix attachment regions during 5th instar posterior silk gland development in Bombyx mori.
  Chunduri AR, Rajan R, Lima A, Ramamoorthy S, Mamillapalli A. Chunduri AR, et al. BMC Genomics. 2022 Mar 31;23(1):247. doi: 10.1186/s12864-022-08446-3. BMC Genomics. 2022. PMID: 35361117 Free PMC article.
• Methylation of Subtelomeric Chromatin Modifies the Expression of the lncRNA TERRA, Disturbing Telomere Homeostasis.
  Oliva-Rico D, Fabian-Morales E, Cáceres-Gutiérrez RE, Gudiño A, Cisneros-Soberanis F, Dominguez J, Almaraz-Rojas O, Arriaga-Canon C, Castro-Hernández C, De la Rosa C, Reyes JL, Herrera LA. Oliva-Rico D, et al. Int J Mol Sci. 2022 Mar 18;23(6):3271. doi: 10.3390/ijms23063271. Int J Mol Sci. 2022. PMID: 35328692 Free PMC article.

References

1. 1. Nucleic Acids Res. 1989 Jun 12;17(11):4235-53 - PubMed
2. 1. Nature. 1989 Apr 27;338(6218):771-4 - PubMed
3. 1. Cell. 1989 Aug 25;58(4):791-801 - PubMed
4. 1. Nucleic Acids Res. 1982 Dec 20;10(24):8145-54 - PubMed
5. 1. Chromosoma. 1981;83(4):541-53 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • Nature Publishing Group
  • PubMed Central
  • Wiley

• Other Literature Sources

  • The Lens - Patent Citations Database

• Research Materials

  • Addgene Non-profit plasmid repository