Detection of Yersinia pestis DNA in two early medieval skeletal finds from Aschheim (Upper Bavaria, 6th century A.D.) - PubMed (original) (raw)
Comparative Study
doi: 10.1002/ajpa.10276.
Affiliations
- PMID: 15386257
- DOI: 10.1002/ajpa.10276
Comparative Study
Detection of Yersinia pestis DNA in two early medieval skeletal finds from Aschheim (Upper Bavaria, 6th century A.D.)
Ingrid Wiechmann et al. Am J Phys Anthropol. 2005 Jan.
Abstract
In the course of a molecular genetic investigation of a double inhumation, presumably a mother/child burial from Aschheim (Upper Bavaria, 6th century A.D.), which included analysis of mitochondrial DNA, molecular sexing, and polymorphic nuclear DNA, Yersinia pestis-specific DNA was detected. Molecular analyses were performed on DNA extracts obtained from two teeth of one skeleton and four teeth of the other. The use of the primer pair YP12D/YP11R (Raoult et al. [2000] Proc. Natl. Acad. Sci. 97:12800-12803), able to amplify part of the Y. pestis plasmid pPCP1 pla sequence, resulted in amplification products of the expected fragment size. Using BLASTN 2.2.2, the sequences of these amplification products shared 100% identity with that of the modern Y. pestis pla sequence in GenBank, with the exception of one amplification product which revealed a single base substitution. The application of a "suicide PCR" with the independent primer pair YP11D/YP10R (Raoult et al. [2000] Proc. Natl. Acad. Sci. 97:12800-12803) resulted in amplification products which shared a 96-98% homology with that of the modern Y. pestis pla sequence in GenBank. The observed deviations were presumably due to miscoding lesions in the template DNA. No modern Y. pestis DNA was introduced into the institute, and thus no positive controls were carried along. All extraction and PCR controls remained negative. The identification of Y. pestis-specific DNA sequences in these two skeletons, buried in the second half of the 6th century A.D., constitutes molecularly supported evidence for the presence of Y. pestis, the causative agent of plague, during the first pandemic recorded.
Similar articles
- Yersinia pestis and the plague of Justinian 541-543 AD: a genomic analysis.
Wagner DM, Klunk J, Harbeck M, Devault A, Waglechner N, Sahl JW, Enk J, Birdsell DN, Kuch M, Lumibao C, Poinar D, Pearson T, Fourment M, Golding B, Riehm JM, Earn DJ, Dewitte S, Rouillard JM, Grupe G, Wiechmann I, Bliska JB, Keim PS, Scholz HC, Holmes EC, Poinar H. Wagner DM, et al. Lancet Infect Dis. 2014 Apr;14(4):319-26. doi: 10.1016/S1473-3099(13)70323-2. Epub 2014 Jan 28. Lancet Infect Dis. 2014. PMID: 24480148 - Molecular identification by "suicide PCR" of Yersinia pestis as the agent of medieval black death.
Raoult D, Aboudharam G, Crubézy E, Larrouy G, Ludes B, Drancourt M. Raoult D, et al. Proc Natl Acad Sci U S A. 2000 Nov 7;97(23):12800-3. doi: 10.1073/pnas.220225197. Proc Natl Acad Sci U S A. 2000. PMID: 11058154 Free PMC article. - Real-time multiplex PCR assay for detection of Yersinia pestis and Yersinia pseudotuberculosis.
Matero P, Pasanen T, Laukkanen R, Tissari P, Tarkka E, Vaara M, Skurnik M. Matero P, et al. APMIS. 2009 Jan;117(1):34-44. doi: 10.1111/j.1600-0463.2008.00013.x. APMIS. 2009. PMID: 19161535 - Targeted enrichment of ancient pathogens yielding the pPCP1 plasmid of Yersinia pestis from victims of the Black Death.
Schuenemann VJ, Bos K, DeWitte S, Schmedes S, Jamieson J, Mittnik A, Forrest S, Coombes BK, Wood JW, Earn DJ, White W, Krause J, Poinar HN. Schuenemann VJ, et al. Proc Natl Acad Sci U S A. 2011 Sep 20;108(38):E746-52. doi: 10.1073/pnas.1105107108. Epub 2011 Aug 29. Proc Natl Acad Sci U S A. 2011. PMID: 21876176 Free PMC article. - [Methods of diagnosis and differentiation of plague pathogen: approaches to detection of atypical strains of Yersinia pestis by molecular biology. Part I].
Trukhachev AL, Lebedeva SA. Trukhachev AL, et al. Mol Gen Mikrobiol Virusol. 2006;(1):3-6. Mol Gen Mikrobiol Virusol. 2006. PMID: 16512602 Review. Russian.
Cited by
- Yersinia pestis: the Natural History of Plague.
Barbieri R, Signoli M, Chevé D, Costedoat C, Tzortzis S, Aboudharam G, Raoult D, Drancourt M. Barbieri R, et al. Clin Microbiol Rev. 2020 Dec 9;34(1):e00044-19. doi: 10.1128/CMR.00044-19. Print 2020 Dec 16. Clin Microbiol Rev. 2020. PMID: 33298527 Free PMC article. Review. - Past pandemics and climate variability across the Mediterranean.
Luterbacher J, Newfield TP, Xoplaki E, Nowatzki E, Luther N, Zhang M, Khelifi N. Luterbacher J, et al. EuroMediterr J Environ Integr. 2020;5(2):46. doi: 10.1007/s41207-020-00197-5. Epub 2020 Sep 19. EuroMediterr J Environ Integr. 2020. PMID: 32984502 Free PMC article. - Ancient dental pulp: Masterpiece tissue for paleomicrobiology.
Mai BHA, Drancourt M, Aboudharam G. Mai BHA, et al. Mol Genet Genomic Med. 2020 Jun;8(6):e1202. doi: 10.1002/mgg3.1202. Epub 2020 Mar 31. Mol Genet Genomic Med. 2020. PMID: 32233019 Free PMC article. Review. - Ancient Yersinia pestis genomes from across Western Europe reveal early diversification during the First Pandemic (541-750).
Keller M, Spyrou MA, Scheib CL, Neumann GU, Kröpelin A, Haas-Gebhard B, Päffgen B, Haberstroh J, Ribera I Lacomba A, Raynaud C, Cessford C, Durand R, Stadler P, Nägele K, Bates JS, Trautmann B, Inskip SA, Peters J, Robb JE, Kivisild T, Castex D, McCormick M, Bos KI, Harbeck M, Herbig A, Krause J. Keller M, et al. Proc Natl Acad Sci U S A. 2019 Jun 18;116(25):12363-12372. doi: 10.1073/pnas.1820447116. Epub 2019 Jun 4. Proc Natl Acad Sci U S A. 2019. PMID: 31164419 Free PMC article. - Yersinia pestis halotolerance illuminates plague reservoirs.
Malek MA, Bitam I, Levasseur A, Terras J, Gaudart J, Azza S, Flaudrops C, Robert C, Raoult D, Drancourt M. Malek MA, et al. Sci Rep. 2017 Jan 5;7:40022. doi: 10.1038/srep40022. Sci Rep. 2017. PMID: 28054667 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources