Cloning and domain structure of the mammalian splicing factor U2AF - PubMed (original) (raw)

. 1992 Feb 13;355(6361):609-14.

doi: 10.1038/355609a0.

Affiliations

• PMID: 1538748
• DOI: 10.1038/355609a0

Cloning and domain structure of the mammalian splicing factor U2AF

P D Zamore et al. Nature. 1992.

Abstract

A complementary DNA clone encoding the large subunit of the essential mammalian pre-messenger RNA splicing component U2 snRNP auxiliary factor (U2AF65) has been isolated and expressed in vitro. It contains two functional domains: a sequence-specific RNA-binding region composed of three ribonucleoprotein-consensus sequence domains, and an arginine/serine-rich motif necessary for splicing but not for binding to pre-mRNA.

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