Rapid eye movement sleep deprivation-induced down-regulation of beta-adrenergic receptors in the rat brainstem and hippocampus - PubMed (original) (raw)
Rapid eye movement sleep deprivation-induced down-regulation of beta-adrenergic receptors in the rat brainstem and hippocampus
Mario Pedrazzoli et al. Pharmacol Biochem Behav. 2004 Sep.
Abstract
Rapid eye movement (REM) sleep deprivation induces a cortical down-regulation of beta-adrenergic receptors. Down-regulation of cortical beta-adrenergic receptors is consistently observed after a number of different chronic antidepressant treatments (drugs and electroconvulsive shock). REM sleep deprivation has an antidepressant effect in humans, and in rats, it decreases immobility in the behavioral despair test, an effect also produced by antidepressant treatments. To verify whether REM sleep deprivation also affects hippocampal beta-adrenergic receptors, we carried out the binding of [3H]-dihydroalprenolol ([3H]-DHA) to hippocampal membranes from rats deprived of REM sleep for 96 h. We also determined the binding of [3H]-DHA to brainstem membranes, a brain region where noradrenergic nuclei are located. Rats were deprived of REM sleep using a water tank with multiple small platforms. [3H-DHA] saturation conditions (concentrations ranging from 0.15 to 6 nM) were obtained in a crude hippocampus and brainstem membrane preparation. Nonspecific binding was determined using DL-propranolol in hippocampus homogenates. In the brainstem homogenates, nonspecific binding was determined in the presence of DL-propranolol or L-isoproterenol. The results obtained showed statistically significant down-regulation of beta-adrenergic receptors in both the hippocampus and the brainstem after REM sleep deprivation. In the hippocampus, there was also a significant decrease in the dissociation constant (KD). In the brainstem, a significant decrease in KD was observed when DL-propranolol was used to determine nonspecific binding. The down-regulation of beta-adrenergic receptors in the hippocampus and brainstem suggests the involvement of these brain areas in the antidepressant effect of REM sleep deprivation.
Similar articles
- Brain distribution of vasoactive intestinal peptide receptors following REM sleep deprivation.
Jiménez-Anguiano A, García-García F, Mendoza-Ramírez JL, Durán- Vázquez A, Drucker-Colín R. Jiménez-Anguiano A, et al. Brain Res. 1996 Jul 22;728(1):37-46. Brain Res. 1996. PMID: 8864295 - Role of alpha and beta adrenoceptors in locus coeruleus stimulation-induced reduction in rapid eye movement sleep in freely moving rats.
Mallick BN, Singh S, Pal D. Mallick BN, et al. Behav Brain Res. 2005 Mar 7;158(1):9-21. doi: 10.1016/j.bbr.2004.08.004. Behav Brain Res. 2005. PMID: 15680190 - Rapid down regulation of beta adrenergic receptors by combining antidepressant drugs with forced swim: a model of antidepressant-induced neural adaptation.
Duncan GE, Paul IA, Harden TK, Mueller RA, Stumpf WE, Breese GR. Duncan GE, et al. J Pharmacol Exp Ther. 1985 Aug;234(2):402-8. J Pharmacol Exp Ther. 1985. PMID: 2991500 - Control of REM sleep: an aspect of the regulation of physiological homeostasis.
Zamboni G, Perez E, Amici R, Jones CA, Parmeggiani PL. Zamboni G, et al. Arch Ital Biol. 1999 Aug;137(4):249-62. Arch Ital Biol. 1999. PMID: 10443317 Review. - Neurobiological bases for the relation between sleep and depression.
Adrien J. Adrien J. Sleep Med Rev. 2002 Oct;6(5):341-51. Sleep Med Rev. 2002. PMID: 12531125 Review.
Cited by
- Neuroendocrine and Peptidergic Regulation of Stress-Induced REM Sleep Rebound.
Machado RB, Suchecki D. Machado RB, et al. Front Endocrinol (Lausanne). 2016 Dec 23;7:163. doi: 10.3389/fendo.2016.00163. eCollection 2016. Front Endocrinol (Lausanne). 2016. PMID: 28066328 Free PMC article. Review. - Clues to the functions of mammalian sleep.
Siegel JM. Siegel JM. Nature. 2005 Oct 27;437(7063):1264-71. doi: 10.1038/nature04285. Nature. 2005. PMID: 16251951 Free PMC article. Review. - Effects of rapid eye movement sleep deprivation on hypocretin neurons in the hypothalamus of a rat model of depression.
Allard JS, Tizabi Y, Shaffery JP, Manaye K. Allard JS, et al. Neuropeptides. 2007 Oct;41(5):329-37. doi: 10.1016/j.npep.2007.04.006. Epub 2007 Jun 27. Neuropeptides. 2007. PMID: 17590434 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources