env mRNAs - PubMed (original) (raw)

The Rev protein of human immunodeficiency virus type 1 promotes polysomal association and translation of gag/pol and vpu/env mRNAs

D M D'Agostino et al. Mol Cell Biol. 1992 Mar.

Abstract

Biochemical examination of the Rev-dependent expression of gag mRNAs produced from gag-Rev-responsive element (RRE) expression plasmids showed a large discrepancy between the level of cytoplasmic gag mRNA and the produced Gag protein. Significant levels of the mRNA produced in the absence of Rev were localized in the cytoplasm, while very low levels of Gag protein were produced. In the presence of Rev, the levels of mRNA increased by 4- to 16-fold, while the Gag protein production increased by 800-fold. These findings indicated that in addition to promoting nucleus-to-cytoplasm transport, Rev increased the utilization of cytoplasmic viral mRNA. Poly(A) selection and in vitro translation of cytoplasmic gag mRNA verified that the mRNA produced in the absence of Rev was functional. To analyze the translational defect in the absence of Rev, we examined the association of the cytoplasmic gag mRNA with ribosomes. gag mRNA produced in the absence of Rev was excluded from polysomes, while gag mRNA produced in the presence of Rev was associated with polysomes and produced Gag protein. These observations showed that the presence of Rev was required for efficient loading of gag mRNA onto polysomes. This effect required the presence of the RRE on the mRNA. Analysis of mRNAs produced from a rev-minus proviral clone confirmed that the presence of Rev promoted polysomal loading of both gag/pol and vpu/env mRNAs. The localization of gag mRNA was also examined by in situ hybridization. This analysis showed that in the presence of Rev, most of the gag mRNA was found in the cytoplasm, while in the absence of Rev, most of the gag mRNA was found in the nucleus and in the region surrounding the nucleus. These results suggest that a substantial fraction of the gag mRNA is retained in distinct cytoplasmic compartments in the absence and presence of Rev. These findings indicate that the presence of Rev is required along the entire mRNA transport and utilization pathway for the stabilization, correct localization, and efficient translation of RRE-containing mRNAs.

PubMed Disclaimer

Cited by

SLBP is associated with histone mRNA on polyribosomes as a component of the histone mRNP.
Whitfield ML, Kaygun H, Erkmann JA, Townley-Tilson WH, Dominski Z, Marzluff WF. Whitfield ML, et al. Nucleic Acids Res. 2004 Sep 9;32(16):4833-42. doi: 10.1093/nar/gkh798. Print 2004. Nucleic Acids Res. 2004. PMID: 15358832 Free PMC article.
The human T-cell leukemia virus Rex protein.
Younis I, Green PL. Younis I, et al. Front Biosci. 2005 Jan 1;10:431-45. doi: 10.2741/1539. Print 2005 Jan 1. Front Biosci. 2005. PMID: 15574380 Free PMC article. Review.
Rev and Rex proteins of human complex retroviruses function with the MMTV Rem-responsive element.
Mertz JA, Lozano MM, Dudley JP. Mertz JA, et al. Retrovirology. 2009 Feb 3;6:10. doi: 10.1186/1742-4690-6-10. Retrovirology. 2009. PMID: 19192308 Free PMC article.
Depletion of hnRNP A2/B1 overrides the nuclear retention of the HIV-1 genomic RNA.
Gordon H, Ajamian L, Valiente-Echeverrìa F, Lévesque K, Rigby WF, Mouland AJ. Gordon H, et al. RNA Biol. 2013 Nov;10(11):1714-25. doi: 10.4161/rna.26542. Epub 2013 Sep 30. RNA Biol. 2013. PMID: 24157614 Free PMC article.
Modulation of HIV-1 gene expression by binding of a ULM motif in the Rev protein to UHM-containing splicing factors.
Pabis M, Corsini L, Vincendeau M, Tripsianes K, Gibson TJ, Brack-Werner R, Sattler M. Pabis M, et al. Nucleic Acids Res. 2019 May 21;47(9):4859-4871. doi: 10.1093/nar/gkz185. Nucleic Acids Res. 2019. PMID: 30892606 Free PMC article.

References

1. J Virol. 1990 Nov;64(11):5448-56 - PubMed
1. Cell. 1980 May;20(1):95-105 - PubMed
1. J Virol. 1990 Jun;64(6):2519-29 - PubMed
1. Proc Natl Acad Sci U S A. 1990 Feb;87(3):1198-202 - PubMed
1. Science. 1990 Feb 16;247(4944):845-8 - PubMed

The Rev protein of human immunodeficiency virus type 1 promotes polysomal association and translation of gag/pol and vpu/env mRNAs - PubMed (original) (raw)