A freeze-frame view of eukaryotic transcription during elongation and capping of nascent mRNA - PubMed (original) (raw)

. 1992 Feb 21;255(5047):983-6.

doi: 10.1126/science.1546295.

Affiliations

• PMID: 1546295
• DOI: 10.1126/science.1546295

A freeze-frame view of eukaryotic transcription during elongation and capping of nascent mRNA

J Hagler et al. Science. 1992.

Abstract

Ribonuclease footprinting of nascent messenger RNA within ternary complexes of vaccinia RNA polymerase revealed an RNA binding site that encompasses an 18-nucleotide RNA segment. The dimensions of the binding site did not change as the polymerase moved along the template. Capping of the 5' end of the RNA was cotranscriptional and was confined to nascent chains 31 nucleotides or greater in length. Purified capping enzyme formed a binary complex with RNA polymerase in solution in the absence of nucleic acid. These findings suggest a mechanism for cotranscriptional establishment of messenger RNA identity in eukaryotes.

PubMed Disclaimer

Similar articles

• Factor-dependent release of nascent RNA by ternary complexes of vaccinia RNA polymerase.
  Deng L, Hagler J, Shuman S. Deng L, et al. J Biol Chem. 1996 Aug 9;271(32):19556-62. doi: 10.1074/jbc.271.32.19556. J Biol Chem. 1996. PMID: 8702649
• Footprinting analysis of mammalian RNA polymerase II along its transcript: an alternative view of transcription elongation.
  Rice GA, Kane CM, Chamberlin MJ. Rice GA, et al. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4245-9. doi: 10.1073/pnas.88.10.4245. Proc Natl Acad Sci U S A. 1991. PMID: 1709738 Free PMC article.
• Interplay of mRNA capping and transcription machineries.
  Kachaev ZM, Lebedeva LA, Kozlov EN, Shidlovskii YV. Kachaev ZM, et al. Biosci Rep. 2020 Jan 31;40(1):BSR20192825. doi: 10.1042/BSR20192825. Biosci Rep. 2020. PMID: 31904821 Free PMC article. Review.
• Cotranscriptional RNA checkpoints.
  de Almeida SF, Carmo-Fonseca M. de Almeida SF, et al. Epigenomics. 2010 Jun;2(3):449-55. doi: 10.2217/epi.10.21. Epigenomics. 2010. PMID: 22121903 Review.

Cited by

• The Vaccinia virion: Filling the gap between atomic and ultrastructure.
  Mirzakhanyan Y, Gershon P. Mirzakhanyan Y, et al. PLoS Pathog. 2019 Jan 7;15(1):e1007508. doi: 10.1371/journal.ppat.1007508. eCollection 2019 Jan. PLoS Pathog. 2019. PMID: 30615658 Free PMC article.
• A dual interface determines the recognition of RNA polymerase II by RNA capping enzyme.
  Suh MH, Meyer PA, Gu M, Ye P, Zhang M, Kaplan CD, Lima CD, Fu J. Suh MH, et al. J Biol Chem. 2010 Oct 29;285(44):34027-38. doi: 10.1074/jbc.M110.145110. Epub 2010 Aug 18. J Biol Chem. 2010. PMID: 20720002 Free PMC article.
• Active site of the mRNA-capping enzyme guanylyltransferase from Saccharomyces cerevisiae: similarity to the nucleotidyl attachment motif of DNA and RNA ligases.
  Fresco LD, Buratowski S. Fresco LD, et al. Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6624-8. doi: 10.1073/pnas.91.14.6624. Proc Natl Acad Sci U S A. 1994. PMID: 8022828 Free PMC article.
• Increased accommodation of nascent RNA in a product site on RNA polymerase II during arrest.
  Gu W, Wind M, Reines D. Gu W, et al. Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):6935-40. doi: 10.1073/pnas.93.14.6935. Proc Natl Acad Sci U S A. 1996. PMID: 8692922 Free PMC article.
• A yeast-based genetic system for functional analysis of viral mRNA capping enzymes.
  Ho CK, Martins A, Shuman S. Ho CK, et al. J Virol. 2000 Jun;74(12):5486-94. doi: 10.1128/jvi.74.12.5486-5494.2000. J Virol. 2000. PMID: 10823853 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon