CTIP1 and CTIP2 are differentially expressed during mouse embryogenesis - PubMed (original) (raw)

CTIP1 and CTIP2 are differentially expressed during mouse embryogenesis

Mark Leid et al. Gene Expr Patterns. 2004 Oct.

Abstract

Chicken ovalbumin upstream promoter transcription factor-interacting proteins 1 and 2 (CTIP1 and CTIP2) are related transcriptional regulatory proteins. While overexpression of both of these proteins has been linked to the development of several lymphoid malignancies, lack of CTIP1 and CTIP2 expression results in defective lymphopoiesis and abnormal thymocyte development, respectively. Here, we describe the expression patterns of CTIP1 and CTIP2 during mouse embryogenesis and in the post-natal brain. Both CTIP1 and CTIP2 were expressed diffusely in the embryo at 10.5 days post-coitum (d.p.c.). However, the expression of both genes became increasingly restricted to the central nervous system (CNS) during the course of fetal development, culminating with high, but differential, expression levels throughout the hippocampal subregions, olfactory bulb and cortex, limbic system, basal ganglia and frontal cortex of the developing brain, and in dorsal cells of the spinal cord. The brain expression domains of CTIP1 and CTIP2 were maintained into adulthood. Outside the CNS, both genes exhibited differential expression within the facial mesenchyme at 12.5 d.p.c., and CTIP2 was selectively expressed from day 12.5 onwards in the olfactory epithelium and developing thymus, and to a lesser extent in oral and gut epithelia. Strong CTIP2 expression was maintained in the thymus at 18.5 d.p.c. These results support the selective contributions of both CTIP1 and CTIP2 in the development and function of both the central nervous and immune systems and the importance of future investigations to define the function(s) of both proteins.

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Figures

Fig. 1

Fig. 1

Expression of CTIP2 and CTIP2 during mouse development. Sagittal sections of mouse embryos or fetuses at 10.5 (A–C), 12.5 (D–F), 14.5 (G–I), and 18.5 d.p.c. (J–L), hybridized with antisense [35S]-labeled cRNAs corresponding to CTIP1 (middle column) and CTIP2 (right column), were examined by dark-field microscopy for signal detection (signal grain appears in white). A bright-field view of one of the corresponding sections is shown in the left column for histological orientation. ba, branchial arches; ce, cerebellum; fb, forebrain; flb, forelimb bud; gu, gut; hb, hindbrain; ht, heart; li, liver; mc, mouth cavity; nc, nasal cavity; ob, olfactory bulb; oe, esophagus; sc, spinal cord; st, striatum; te, telencephalic vesicle; th, thymus. Arrowheads in (E,F) point to differential spatial expression domains in the developing mandibular and frontonasal region.

Fig. 2

Fig. 2

Expression of CTIP1 and CTIP2 in the developing mouse brain. Embryos were sagittally sectioned at 14.5 (A–F) and 18.5 d.p.c. (G–L), and hybridized with [35S]-labeled probes as indicated. ap, anterior pituitary; bg, basal ganglia; cc, cerebral cortex; ce, cerebellum; di, diencephalon; g5, 5th cranial nerve ganglion (trigeminal ganglion); hi, hippocampus; ht, hypothalamus; ic, inferior colliculus; me, mesencephalon; nc, nasal cavity; ob, olfactory bulb; po, pontine nucleus.

Fig. 3

Fig. 3

Mouse brain expression of CTIP1 and CTIP2 at post-natal day 21. Mouse brain coronal sections from post-natal day 21 animals were probed with [35S]-labeled riboprobes as indicated. A bright-field image is shown in the left column for histological orientation of the in situ hybridization panels (dark-field images in the middle and right columns). The sections shown are progressively more caudal from the top to the bottom rows. an, amydaloid nucleus; cc, cerebral cortex; cp, caudate-putamen; dg, dentate gyrus; hi, hippocampus; ht, hypothalamus, lateral septal nucleus; oc, olfactory cortex; pv, paraventricular thalamic nucleus; th, thalamus.

Fig. 4

Fig. 4

Expression of CTIP1 and CTIP2 in adult cerebral cortex, hippocampus, and cerebellum. Coronal cryosections of adult mouse brain were probed with [35S]-labeled CTIP1 (B) and CTIP2 (C) riboprobes. (A) Bright-field image for panels B and C. (D–F) Sagittal sections of adult mouse cerebellum hybridized with CTIP1 (E) and CTIP2 (F) probes and the corresponding bright-field image (D). CA1–CA4, hippocampal subregions; cc, cerebral cortex; hi, hippocampus; dg, dentate gyrus; th, thalamus; pl, Purkinje cell layer.

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