Interaction of the dye ethidium bromide with DNA containing guanine repeats - PubMed (original) (raw)
. 1992 Mar 10;31(9):2451-5.
doi: 10.1021/bi00124a002.
Affiliations
- PMID: 1547228
- DOI: 10.1021/bi00124a002
Interaction of the dye ethidium bromide with DNA containing guanine repeats
Q Guo et al. Biochemistry. 1992.
Abstract
DNA containing one or more copies of the motifs repeated in telomere sequences has unusual conformational properties. The isolated sequence from the protozoan Oxytricha, dT4G4 has the potential to form tetramers in the presence of sodium or potassium ions. We report here that these tetramers bind ethidium tightly, with an interaction that fulfills several criteria for an intercalative mechanism in the G sequence. By contrast, the 4-fold tandem repeat of this subunit, d(T4G4)4, does not interact specifically with ethidium in the presence of Na+. This difference might have a simple structural basis: the tetramer of dT4G4 forms a stack of four G-quartets in the presence of Na+ or K+, whereas the constraint imposed by the T4 "tethers" in the repeat d(T4G4)4 allows only two layers to form in the presence of Na+. In the presence of sufficient K+, the latter can partially form a four-layer G-quartet structure, which interacts with ethidium. This idea is supported by analysis of a "relaxed" sequence, dT4G4(T7G4)3, which allows formation of four G-quartets and binds ethidium in the presence of Na+ as well as K+. Ethidium (and intercalators generally) should thus be able to retard or inhibit the action of telomerase in the presence of K+.
Similar articles
- The effect of sodium, potassium and ammonium ions on the conformation of the dimeric quadruplex formed by the Oxytricha nova telomere repeat oligonucleotide d(G(4)T(4)G(4)).
Schultze P, Hud NV, Smith FW, Feigon J. Schultze P, et al. Nucleic Acids Res. 1999 Aug 1;27(15):3018-28. doi: 10.1093/nar/27.15.3018. Nucleic Acids Res. 1999. PMID: 10454595 Free PMC article. - Structure and stability of sodium and potassium complexes of dT4G4 and dT4G4T.
Lu M, Guo Q, Kallenbach NR. Lu M, et al. Biochemistry. 1992 Mar 10;31(9):2455-9. doi: 10.1021/bi00124a003. Biochemistry. 1992. PMID: 1547229 - Structural basis of DNA recognition and mechanism of quadruplex formation by the beta subunit of the Oxytricha telomere binding protein.
Laporte L, Thomas GJ Jr. Laporte L, et al. Biochemistry. 1998 Feb 3;37(5):1327-35. doi: 10.1021/bi972400d. Biochemistry. 1998. PMID: 9477960 - Molecular determinants for DNA minor groove recognition: design of a bis-guanidinium derivative of ethidium that is highly selective for AT-rich DNA sequences.
Bailly C, Arafa RK, Tanious FA, Laine W, Tardy C, Lansiaux A, Colson P, Boykin DW, Wilson WD. Bailly C, et al. Biochemistry. 2005 Feb 15;44(6):1941-52. doi: 10.1021/bi047983n. Biochemistry. 2005. PMID: 15697219 - The structure of the aflatoxin B1-DNA adduct at N7 of guanine. Theoretical intercalation and covalent adduct models.
Bonnett M, Taylor ER. Bonnett M, et al. J Biomol Struct Dyn. 1989 Aug;7(1):127-49. doi: 10.1080/07391102.1989.10507756. J Biomol Struct Dyn. 1989. PMID: 2510767 Review.
Cited by
- New Xanthone Derivatives as Potent G-Quadruplex Binders for Developing Anti-Cancer Therapeutics.
Roy S, Maiti B, Banerjee N, Kaulage MH, Muniyappa K, Chatterjee S, Bhattacharya S. Roy S, et al. ACS Pharmacol Transl Sci. 2023 Mar 24;6(4):546-566. doi: 10.1021/acsptsci.2c00205. eCollection 2023 Apr 14. ACS Pharmacol Transl Sci. 2023. PMID: 37082748 Free PMC article. - Calix[4]arene Polyamine Triazoles: Synthesis, Aggregation and DNA Binding.
Burilov V, Makarov E, Mironova D, Sultanova E, Bilyukova I, Akyol K, Evtugyn V, Islamov D, Usachev K, Mukhametzyanov T, Solovieva S, Antipin I. Burilov V, et al. Int J Mol Sci. 2022 Nov 28;23(23):14889. doi: 10.3390/ijms232314889. Int J Mol Sci. 2022. PMID: 36499212 Free PMC article. - Interactions of small molecules with DNA junctions.
McQuaid KT, Pipier A, Cardin CJ, Monchaud D. McQuaid KT, et al. Nucleic Acids Res. 2022 Dec 9;50(22):12636-12656. doi: 10.1093/nar/gkac1043. Nucleic Acids Res. 2022. PMID: 36382400 Free PMC article. Review. - Targeting of Telomeric Repeat-Containing RNA G-Quadruplexes: From Screening to Biophysical and Biological Characterization of a New Hit Compound.
Marzano S, Pagano B, Iaccarino N, Di Porzio A, De Tito S, Vertecchi E, Salvati E, Randazzo A, Amato J. Marzano S, et al. Int J Mol Sci. 2021 Sep 24;22(19):10315. doi: 10.3390/ijms221910315. Int J Mol Sci. 2021. PMID: 34638655 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources