Cytokine secretion from mononuclear cells cultured in vitro with starch-based polymers and poly-L-lactide - PubMed (original) (raw)
. 2004 Dec 1;71(3):419-29.
doi: 10.1002/jbm.a.30155.
Affiliations
- PMID: 15472922
- DOI: 10.1002/jbm.a.30155
Cytokine secretion from mononuclear cells cultured in vitro with starch-based polymers and poly-L-lactide
Alexandra P Marques et al. J Biomed Mater Res A. 2004.
Abstract
The cytokine network is one of the major controlling systems of the inflammatory process, driving the magnitude and duration of the host response against invading microorganisms, foreign materials, or altered internal stimuli. Pro- and antiinflammatory cytokines were quantified after in vitro culture of a mixed population of monocytes/macrophages and lymphocytes with biodegradable polymers. Different blends of starch-based polymers and their composites filled with hydroxyapatite were studied and compared with poly-L-lactide. Interleukin (IL)-1beta, IL-6, and tumour necrosis factor-alpha were investigated as the markers of immunological reactivity because they are known to act at the early stages of injury/invasion. Interferon-gamma, recognized as a proinflammatory cytokine, although not present during early responses was also investigated. Contrarily, IL-4 derived from T lymphocytes, was investigated because it is an immunoregulator that counteracts some aspects of inflammation. T lymphocyte activation was also determined by quantifying IL-2. The results support the hypothesis that different biodegradable polymers can affect mononuclear cell activation and the production of several cytokines associated with the inflammatory process. No IL-2 or interferon-gamma was found in the culture supernatants after 3, 7, and 14 days in the presence of any of the materials. IL-6 was detected in the highest amounts, for all the conditions, followed by tumour necrosis factor-alpha. IL-1beta was produced in very low amounts, being undetectable with some of the starch-based materials. IL-4 was the only cytokine that did not demonstrate any significant difference within this group of materials. Starch-based polymers and composites induced lower production of proinflammatory cytokines in comparison to poly-L-lactide.
Similar articles
- Cytokine response to lipoprotein lipid loading in human monocyte-derived macrophages.
Persson J, Nilsson J, Lindholm MW. Persson J, et al. Lipids Health Dis. 2006 Jun 26;5:17. doi: 10.1186/1476-511X-5-17. Lipids Health Dis. 2006. PMID: 16800873 Free PMC article. - Adhesion and cytokine production by monocytes on poly(2-methacryloyloxyethyl phosphorylcholine-co-alkyl methacrylate)-coated polymers.
DeFife KM, Yun JK, Azeez A, Stack S, Ishihara K, Nakabayashi N, Colton E, Anderson JM. DeFife KM, et al. J Biomed Mater Res. 1995 Apr;29(4):431-9. doi: 10.1002/jbm.820290403. J Biomed Mater Res. 1995. PMID: 7622528 - Evaluation of inflammatory cytokine secretion by human alveolar macrophages.
Losa García JE, Rodríguez FM, Martín de Cabo MR, García Salgado MJ, Losada JP, Villarón LG, López AJ, Arellano JL. Losa García JE, et al. Mediators Inflamm. 1999;8(1):43-51. doi: 10.1080/09629359990711. Mediators Inflamm. 1999. PMID: 10704089 Free PMC article. - Mast cell activation and its relation to proinflammatory cytokine production in the rheumatoid lesion.
Woolley DE, Tetlow LC. Woolley DE, et al. Arthritis Res. 2000;2(1):65-74. doi: 10.1186/ar70. Arthritis Res. 2000. PMID: 11219391 Free PMC article. - Impact of 3-D printed PLA- and chitosan-based scaffolds on human monocyte/macrophage responses: unraveling the effect of 3-D structures on inflammation.
Almeida CR, Serra T, Oliveira MI, Planell JA, Barbosa MA, Navarro M. Almeida CR, et al. Acta Biomater. 2014 Feb;10(2):613-22. doi: 10.1016/j.actbio.2013.10.035. Epub 2013 Nov 5. Acta Biomater. 2014. PMID: 24211731
Cited by
- Rational design of hydrogels for immunomodulation.
Bu W, Wu Y, Ghaemmaghami AM, Sun H, Mata A. Bu W, et al. Regen Biomater. 2022 Feb 22;9:rbac009. doi: 10.1093/rb/rbac009. eCollection 2022. Regen Biomater. 2022. PMID: 35668923 Free PMC article. Review. - Natural Polysaccharide Nanomaterials: An Overview of Their Immunological Properties.
Torres FG, Troncoso OP, Pisani A, Gatto F, Bardi G. Torres FG, et al. Int J Mol Sci. 2019 Oct 14;20(20):5092. doi: 10.3390/ijms20205092. Int J Mol Sci. 2019. PMID: 31615111 Free PMC article. Review. - Treatment of Mycobacterium tuberculosis-Infected Macrophages with Poly(Lactic-Co-Glycolic Acid) Microparticles Drives NFκB and Autophagy Dependent Bacillary Killing.
Lawlor C, O'Connor G, O'Leary S, Gallagher PJ, Cryan SA, Keane J, O'Sullivan MP. Lawlor C, et al. PLoS One. 2016 Feb 19;11(2):e0149167. doi: 10.1371/journal.pone.0149167. eCollection 2016. PLoS One. 2016. PMID: 26894562 Free PMC article. - pH-Responsive Isoniazid-Loaded Nanoparticles Markedly Improve Tuberculosis Treatment in Mice.
Hwang AA, Lee BY, Clemens DL, Dillon BJ, Zink JI, Horwitz MA. Hwang AA, et al. Small. 2015 Oct;11(38):5066-78. doi: 10.1002/smll.201500937. Epub 2015 Jul 20. Small. 2015. PMID: 26193431 Free PMC article. - Safety of poly (ethylene glycol)-coated perfluorodecalin-filled poly (lactide-co-glycolide) microcapsules following intravenous administration of high amounts in rats.
Ferenz KB, Waack IN, Laudien J, Mayer C, Broecker-Preuss M, Groot Hd, Kirsch M. Ferenz KB, et al. Results Pharma Sci. 2014 Apr 30;4:8-18. doi: 10.1016/j.rinphs.2014.04.001. eCollection 2014. Results Pharma Sci. 2014. PMID: 25756002 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources