A conserved interaction between the replicative clamp loader and DNA ligase in eukaryotes: implications for Okazaki fragment joining - PubMed (original) (raw)
. 2004 Dec 31;279(53):55196-201.
doi: 10.1074/jbc.M409250200. Epub 2004 Oct 23.
Affiliations
- PMID: 15502161
- DOI: 10.1074/jbc.M409250200
Free article
A conserved interaction between the replicative clamp loader and DNA ligase in eukaryotes: implications for Okazaki fragment joining
David S Levin et al. J Biol Chem. 2004.
Free article
Abstract
The recruitment of DNA ligase I to replication foci and the efficient joining of Okazaki fragments is dependent on the interaction between DNA ligase I and proliferating cell nuclear antigen (PCNA). Although the PCNA sliding clamp tethers DNA ligase I to nicked duplex DNA circles, the interaction does not enhance DNA joining. This suggests that other factors may be involved in the joining of Okazaki fragments. In this study, we describe an association between replication factor C (RFC), the clamp loader, and DNA ligase I in human cell extracts. Subsequently, we demonstrate that there is a direct physical interaction between these proteins that involves both the N- and C-terminal domains of DNA ligase I, the N terminus of the large RFC subunit p140, and the p36 and p38 subunits of RFC. Although RFC inhibited DNA joining by DNA ligase I, the addition of PCNA alleviated inhibition by RFC. Notably, the effect of PCNA on ligation was dependent on the PCNA-binding site of DNA ligase I. Together, these results provide a molecular explanation for the key in vivo role of the DNA ligase I/PCNA interaction and suggest that the joining of Okazaki fragments is coordinated by pairwise interactions among RFC, PCNA, and DNA ligase I.
Similar articles
- The C-terminal domain of yeast PCNA is required for physical and functional interactions with Cdc9 DNA ligase.
Vijayakumar S, Chapados BR, Schmidt KH, Kolodner RD, Tainer JA, Tomkinson AE. Vijayakumar S, et al. Nucleic Acids Res. 2007;35(5):1624-37. doi: 10.1093/nar/gkm006. Epub 2007 Feb 18. Nucleic Acids Res. 2007. PMID: 17308348 Free PMC article. - A conserved physical and functional interaction between the cell cycle checkpoint clamp loader and DNA ligase I of eukaryotes.
Song W, Levin DS, Varkey J, Post S, Bermudez VP, Hurwitz J, Tomkinson AE. Song W, et al. J Biol Chem. 2007 Aug 3;282(31):22721-30. doi: 10.1074/jbc.M703774200. Epub 2007 Jun 8. J Biol Chem. 2007. PMID: 17561505 - An interaction between DNA ligase I and proliferating cell nuclear antigen: implications for Okazaki fragment synthesis and joining.
Levin DS, Bai W, Yao N, O'Donnell M, Tomkinson AE. Levin DS, et al. Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):12863-8. doi: 10.1073/pnas.94.24.12863. Proc Natl Acad Sci U S A. 1997. PMID: 9371766 Free PMC article. - Functions of Multiple Clamp and Clamp-Loader Complexes in Eukaryotic DNA Replication.
Ohashi E, Tsurimoto T. Ohashi E, et al. Adv Exp Med Biol. 2017;1042:135-162. doi: 10.1007/978-981-10-6955-0_7. Adv Exp Med Biol. 2017. PMID: 29357057 Review. - DNA ligase I, the replicative DNA ligase.
Howes TR, Tomkinson AE. Howes TR, et al. Subcell Biochem. 2012;62:327-41. doi: 10.1007/978-94-007-4572-8_17. Subcell Biochem. 2012. PMID: 22918593 Free PMC article. Review.
Cited by
- DNA ligase I variants fail in the ligation of mutagenic repair intermediates with mismatches and oxidative DNA damage.
Tang Q, Kamble P, Çağlayan M. Tang Q, et al. Mutagenesis. 2020 Dec 1;35(5):391-404. doi: 10.1093/mutage/geaa023. Mutagenesis. 2020. PMID: 32914844 Free PMC article. - Reconstitution of eukaryotic lagging strand DNA replication.
Balakrishnan L, Gloor JW, Bambara RA. Balakrishnan L, et al. Methods. 2010 Jul;51(3):347-57. doi: 10.1016/j.ymeth.2010.02.017. Epub 2010 Feb 21. Methods. 2010. PMID: 20178844 Free PMC article. Review. - The C-terminal domain of yeast PCNA is required for physical and functional interactions with Cdc9 DNA ligase.
Vijayakumar S, Chapados BR, Schmidt KH, Kolodner RD, Tainer JA, Tomkinson AE. Vijayakumar S, et al. Nucleic Acids Res. 2007;35(5):1624-37. doi: 10.1093/nar/gkm006. Epub 2007 Feb 18. Nucleic Acids Res. 2007. PMID: 17308348 Free PMC article. - Proliferating cell nuclear antigen restores the enzymatic activity of a DNA ligase I deficient in DNA binding.
Trasviña-Arenas CH, Cardona-Felix CS, Azuara-Liceaga E, Díaz-Quezada C, Brieba LG. Trasviña-Arenas CH, et al. FEBS Open Bio. 2017 Mar 16;7(5):659-674. doi: 10.1002/2211-5463.12209. eCollection 2017 May. FEBS Open Bio. 2017. PMID: 28469979 Free PMC article. - Control of Genome Integrity by RFC Complexes; Conductors of PCNA Loading onto and Unloading from Chromatin during DNA Replication.
Shiomi Y, Nishitani H. Shiomi Y, et al. Genes (Basel). 2017 Jan 26;8(2):52. doi: 10.3390/genes8020052. Genes (Basel). 2017. PMID: 28134787 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous