alpha2-Macroglobulin-proteinase complexes protect Streptococcus pyogenes from killing by the antimicrobial peptide LL-37 - PubMed (original) (raw)
. 2004 Dec 17;279(51):52820-3.
doi: 10.1074/jbc.C400485200. Epub 2004 Nov 1.
Affiliations
- PMID: 15520011
- DOI: 10.1074/jbc.C400485200
Free article
alpha2-Macroglobulin-proteinase complexes protect Streptococcus pyogenes from killing by the antimicrobial peptide LL-37
Patrik Nyberg et al. J Biol Chem. 2004.
Free article
Abstract
The significant human bacterial pathogen Streptococcus pyogenes expresses GRAB, a surface protein that binds alpha(2)-macroglobulin (alpha(2)M), a major proteinase inhibitor of human plasma. alpha(2)M inhibits proteolysis by trapping the proteinase, which, however, still remains proteolytically active against smaller peptides that can penetrate the alpha(2)M-proteinase complex. Here we report that SpeB, a cysteine proteinase secreted by S. pyogenes, is trapped by alpha(2)M bound to protein GRAB. As a consequence, SpeB is retained at the bacterial surface and protects S. pyogenes against killing by the antibacterial peptide LL-37.
Similar articles
- Protein GRAB of streptococcus pyogenes regulates proteolysis at the bacterial surface by binding alpha2-macroglobulin.
Rasmussen M, Müller HP, Björck L. Rasmussen M, et al. J Biol Chem. 1999 May 28;274(22):15336-44. doi: 10.1074/jbc.274.22.15336. J Biol Chem. 1999. PMID: 10336419 - Cathelicidin LL-37 in severe Streptococcus pyogenes soft tissue infections in humans.
Johansson L, Thulin P, Sendi P, Hertzén E, Linder A, Akesson P, Low DE, Agerberth B, Norrby-Teglund A. Johansson L, et al. Infect Immun. 2008 Aug;76(8):3399-404. doi: 10.1128/IAI.01392-07. Epub 2008 May 19. Infect Immun. 2008. PMID: 18490458 Free PMC article. - IdeS, a secreted proteinase of Streptococcus pyogenes, is bound to a nuclease at the bacterial surface where it inactivates opsonizing IgG antibodies.
Frick IM, Happonen L, Wrighton S, Nordenfelt P, Björck L. Frick IM, et al. J Biol Chem. 2023 Nov;299(11):105345. doi: 10.1016/j.jbc.2023.105345. Epub 2023 Oct 12. J Biol Chem. 2023. PMID: 37838172 Free PMC article. - Cysteine proteinase SpeB from Streptococcus pyogenes - a potent modifier of immunologically important host and bacterial proteins.
Nelson DC, Garbe J, Collin M. Nelson DC, et al. Biol Chem. 2011 Dec;392(12):1077-88. doi: 10.1515/BC.2011.208. Biol Chem. 2011. PMID: 22050223 Review. - IdeS and SpeB: immunoglobulin-degrading cysteine proteinases of Streptococcus pyogenes.
von Pawel-Rammingen U, Björck L. von Pawel-Rammingen U, et al. Curr Opin Microbiol. 2003 Feb;6(1):50-5. doi: 10.1016/s1369-5274(03)00003-1. Curr Opin Microbiol. 2003. PMID: 12615219 Review.
Cited by
- Molecular Mechanisms of Bacterial Resistance to Antimicrobial Peptides in the Modern Era: An Updated Review.
Tajer L, Paillart JC, Dib H, Sabatier JM, Fajloun Z, Abi Khattar Z. Tajer L, et al. Microorganisms. 2024 Jun 21;12(7):1259. doi: 10.3390/microorganisms12071259. Microorganisms. 2024. PMID: 39065030 Free PMC article. Review. - pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination.
Boukthir S, Gaudu P, Faili A, Kayal S. Boukthir S, et al. Heliyon. 2023 May 29;9(6):e16720. doi: 10.1016/j.heliyon.2023.e16720. eCollection 2023 Jun. Heliyon. 2023. PMID: 37346331 Free PMC article. - Immunomodulatory and Allergenic Properties of Antimicrobial Peptides.
Guryanova SV, Ovchinnikova TV. Guryanova SV, et al. Int J Mol Sci. 2022 Feb 24;23(5):2499. doi: 10.3390/ijms23052499. Int J Mol Sci. 2022. PMID: 35269641 Free PMC article. Review. - Proteolytic Profiling of Streptococcal Pyrogenic Exotoxin B (SpeB) by Complementary HPLC-MS Approaches.
Blöchl C, Holzner C, Luciano M, Bauer R, Horejs-Hoeck J, Eckhard U, Brandstetter H, Huber CG. Blöchl C, et al. Int J Mol Sci. 2021 Dec 30;23(1):412. doi: 10.3390/ijms23010412. Int J Mol Sci. 2021. PMID: 35008838 Free PMC article. - Alpha-2-Macroglobulin in Inflammation, Immunity and Infections.
Vandooren J, Itoh Y. Vandooren J, et al. Front Immunol. 2021 Dec 14;12:803244. doi: 10.3389/fimmu.2021.803244. eCollection 2021. Front Immunol. 2021. PMID: 34970276 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials