Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits - PubMed (original) (raw)
. 2004 Dec 31;279(53):55411-8.
doi: 10.1074/jbc.M406876200. Epub 2004 Nov 3.
Affiliations
- PMID: 15528184
- DOI: 10.1074/jbc.M406876200
Free article
Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits
Kyriaki Galani et al. J Biol Chem. 2004.
Free article
Abstract
Rea1, the largest predicted protein in the yeast genome, is a member of the AAA(+) family of ATPases and is associated with pre-60 S ribosomes. Here we report that Rea1 is required for maturation and nuclear export of the pre-60 S subunit. Rea1 exhibits a predominantly nucleoplasmic localization and is present in a late pre-60 S particle together with members of the Rix1 complex. To study the role of Rea1 in ribosome biogenesis, we generated a repressible GAL::REA1 strain and temperature-sensitive rea1 alleles. In vivo depletion of Rea1 results in the significant reduction of mature 60 S subunits concomitant with defects in pre-rRNA processing and late pre-60 S ribosome stability following ITS2 cleavage and prior to the generation of mature 5.8 S rRNA. Strains depleted of the components of the Rix1 complex (Rix1, Ipi1, and Ipi3) showed similar defects. Using an in vivo 60 S subunit export assay, a strong accumulation of the large subunit reporter Rpl25-GFP (green fluorescent protein) in the nucleus and at the nuclear periphery was seen in rea1 mutants at restrictive conditions.
Similar articles
- Architecture of the Rix1-Rea1 checkpoint machinery during pre-60S-ribosome remodeling.
Barrio-Garcia C, Thoms M, Flemming D, Kater L, Berninghausen O, Baßler J, Beckmann R, Hurt E. Barrio-Garcia C, et al. Nat Struct Mol Biol. 2016 Jan;23(1):37-44. doi: 10.1038/nsmb.3132. Epub 2015 Nov 30. Nat Struct Mol Biol. 2016. PMID: 26619264 - A pre-ribosome with a tadpole-like structure functions in ATP-dependent maturation of 60S subunits.
Nissan TA, Galani K, Maco B, Tollervey D, Aebi U, Hurt E. Nissan TA, et al. Mol Cell. 2004 Jul 23;15(2):295-301. doi: 10.1016/j.molcel.2004.06.033. Mol Cell. 2004. PMID: 15260980 - Coupled GTPase and remodelling ATPase activities form a checkpoint for ribosome export.
Matsuo Y, Granneman S, Thoms M, Manikas RG, Tollervey D, Hurt E. Matsuo Y, et al. Nature. 2014 Jan 2;505(7481):112-116. doi: 10.1038/nature12731. Epub 2013 Nov 17. Nature. 2014. PMID: 24240281 Free PMC article. - Nuclear export and cytoplasmic maturation of ribosomal subunits.
Zemp I, Kutay U. Zemp I, et al. FEBS Lett. 2007 Jun 19;581(15):2783-93. doi: 10.1016/j.febslet.2007.05.013. Epub 2007 May 11. FEBS Lett. 2007. PMID: 17509569 Review. - [Quality control of pre-ribosome coupled with ribosome biogenesis].
Matsuo Y. Matsuo Y. Seikagaku. 2014 Dec;86(6):793-7. Seikagaku. 2014. PMID: 25675820 Review. Japanese. No abstract available.
Cited by
- Analysis of ribosome biogenesis factor-modules in yeast cells depleted from pre-ribosomes.
Merl J, Jakob S, Ridinger K, Hierlmeier T, Deutzmann R, Milkereit P, Tschochner H. Merl J, et al. Nucleic Acids Res. 2010 May;38(9):3068-80. doi: 10.1093/nar/gkp1244. Epub 2010 Jan 25. Nucleic Acids Res. 2010. PMID: 20100801 Free PMC article. - The power of AAA-ATPases on the road of pre-60S ribosome maturation--molecular machines that strip pre-ribosomal particles.
Kressler D, Hurt E, Bergler H, Bassler J. Kressler D, et al. Biochim Biophys Acta. 2012 Jan;1823(1):92-100. doi: 10.1016/j.bbamcr.2011.06.017. Epub 2011 Jul 5. Biochim Biophys Acta. 2012. PMID: 21763358 Free PMC article. Review. - The essential WD-repeat protein Rsa4p is required for rRNA processing and intra-nuclear transport of 60S ribosomal subunits.
de la Cruz J, Sanz-Martínez E, Remacha M. de la Cruz J, et al. Nucleic Acids Res. 2005 Oct 12;33(18):5728-39. doi: 10.1093/nar/gki887. Print 2005. Nucleic Acids Res. 2005. PMID: 16221974 Free PMC article. - The MIDASIN and NOTCHLESS genes are essential for female gametophyte development in Arabidopsis thaliana.
Chantha SC, Gray-Mitsumune M, Houde J, Matton DP. Chantha SC, et al. Physiol Mol Biol Plants. 2010 Jan;16(1):3-18. doi: 10.1007/s12298-010-0005-y. Epub 2010 Aug 13. Physiol Mol Biol Plants. 2010. PMID: 23572950 Free PMC article. - The splicing ATPase prp43p is a component of multiple preribosomal particles.
Lebaron S, Froment C, Fromont-Racine M, Rain JC, Monsarrat B, Caizergues-Ferrer M, Henry Y. Lebaron S, et al. Mol Cell Biol. 2005 Nov;25(21):9269-82. doi: 10.1128/MCB.25.21.9269-9282.2005. Mol Cell Biol. 2005. PMID: 16227579 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases