Insights into the mRNA cleavage mechanism by MazF, an mRNA interferase - PubMed (original) (raw)
. 2005 Feb 4;280(5):3143-50.
doi: 10.1074/jbc.M411811200. Epub 2004 Nov 10.
Affiliations
- PMID: 15537630
- DOI: 10.1074/jbc.M411811200
Free article
Insights into the mRNA cleavage mechanism by MazF, an mRNA interferase
Yonglong Zhang et al. J Biol Chem. 2005.
Free article
Abstract
MazF is an Escherichia coli toxin that is highly conserved among the prokaryotes and plays an important role in growth regulation. When MazF is induced, protein synthesis is effectively inhibited. However, the mechanism of MazF action has been controversial. Here we unequivocally demonstrate that MazF is an endoribonuclease that specifically cleaves mRNAs at ACA sequences. We then demonstrate its enzymatic specificity using short RNA substrates. MazF cleaves RNA at the 5'-end of ACA sequences, yielding a 2',3'-cyclic phosphate at one side and a free 5'-OH group at the other. Using DNA-RNA chimeric substrates containing XACA, the 2'-OH group of residue X was found absolutely essential for MazF cleavage, whereas all the other residues may be deoxyriboses. Therefore, MazF exhibits exquisite site specificity and has utility as an RNA-restriction enzyme for RNA structural studies or as an mRNA interferase to regulate cell growth in prokaryotic and eukaryotic cells.
Similar articles
- ACA-specific RNA sequence recognition is acquired via the loop 2 region of MazF mRNA interferase.
Park JH, Yoshizumi S, Yamaguchi Y, Wu KP, Inouye M. Park JH, et al. Proteins. 2013 May;81(5):874-83. doi: 10.1002/prot.24246. Epub 2013 Feb 25. Proteins. 2013. PMID: 23280569 Free PMC article. - Characterization of ChpBK, an mRNA interferase from Escherichia coli.
Zhang Y, Zhu L, Zhang J, Inouye M. Zhang Y, et al. J Biol Chem. 2005 Jul 15;280(28):26080-8. doi: 10.1074/jbc.M502050200. Epub 2005 May 18. J Biol Chem. 2005. PMID: 15901733 - MazF cleaves cellular mRNAs specifically at ACA to block protein synthesis in Escherichia coli.
Zhang Y, Zhang J, Hoeflich KP, Ikura M, Qing G, Inouye M. Zhang Y, et al. Mol Cell. 2003 Oct;12(4):913-23. doi: 10.1016/s1097-2765(03)00402-7. Mol Cell. 2003. PMID: 14580342 - The discovery of mRNA interferases: implication in bacterial physiology and application to biotechnology.
Inouye M. Inouye M. J Cell Physiol. 2006 Dec;209(3):670-6. doi: 10.1002/jcp.20801. J Cell Physiol. 2006. PMID: 17001682 Review. - mRNA interferases, sequence-specific endoribonucleases from the toxin-antitoxin systems.
Yamaguchi Y, Inouye M. Yamaguchi Y, et al. Prog Mol Biol Transl Sci. 2009;85:467-500. doi: 10.1016/S0079-6603(08)00812-X. Prog Mol Biol Transl Sci. 2009. PMID: 19215780 Review.
Cited by
- The RNA ligase RtcB reverses MazF-induced ribosome heterogeneity in Escherichia coli.
Temmel H, Müller C, Sauert M, Vesper O, Reiss A, Popow J, Martinez J, Moll I. Temmel H, et al. Nucleic Acids Res. 2017 May 5;45(8):4708-4721. doi: 10.1093/nar/gkw1018. Nucleic Acids Res. 2017. PMID: 27789694 Free PMC article. - RtcB is the RNA ligase component of an Escherichia coli RNA repair operon.
Tanaka N, Shuman S. Tanaka N, et al. J Biol Chem. 2011 Mar 11;286(10):7727-7731. doi: 10.1074/jbc.C111.219022. Epub 2011 Jan 11. J Biol Chem. 2011. PMID: 21224389 Free PMC article. - The communication factor EDF and the toxin-antitoxin module mazEF determine the mode of action of antibiotics.
Kolodkin-Gal I, Sat B, Keshet A, Engelberg-Kulka H. Kolodkin-Gal I, et al. PLoS Biol. 2008 Dec 16;6(12):e319. doi: 10.1371/journal.pbio.0060319. PLoS Biol. 2008. PMID: 19090622 Free PMC article. - Quorum Sensing Extracellular Death Peptides Enhance the Endoribonucleolytic Activities of Mycobacterium tuberculosis MazF Toxins.
Nigam A, Kumar S, Engelberg-Kulka H. Nigam A, et al. mBio. 2018 May 1;9(3):e00685-18. doi: 10.1128/mBio.00685-18. mBio. 2018. PMID: 29717013 Free PMC article. - Transition State Charge Stabilization and Acid-Base Catalysis of mRNA Cleavage by the Endoribonuclease RelE.
Dunican BF, Hiller DA, Strobel SA. Dunican BF, et al. Biochemistry. 2015 Dec 1;54(47):7048-57. doi: 10.1021/acs.biochem.5b00866. Epub 2015 Nov 12. Biochemistry. 2015. PMID: 26535789 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources