Multidimensional drug profiling by automated microscopy - PubMed (original) (raw)

. 2004 Nov 12;306(5699):1194-8.

doi: 10.1126/science.1100709.

Affiliations

• PMID: 15539606
• DOI: 10.1126/science.1100709

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Multidimensional drug profiling by automated microscopy

Zachary E Perlman et al. Science. 2004.

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Abstract

We present a method for high-throughput cytological profiling by microscopy. Our system provides quantitative multidimensional measures of individual cell states over wide ranges of perturbations. We profile dose-dependent phenotypic effects of drugs in human cell culture with a titration-invariant similarity score (TISS). This method successfully categorized blinded drugs and suggested targets for drugs of uncertain mechanism. Multivariate single-cell analysis is a starting point for identifying relationships among drug effects at a systems level and a step toward phenotypic profiling at the single-cell level. Our methods will be useful for discovering the mechanism and predicting the toxicity of new drugs.

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