No transcription-translation feedback in circadian rhythm of KaiC phosphorylation - PubMed (original) (raw)

. 2005 Jan 14;307(5707):251-4.

doi: 10.1126/science.1102540. Epub 2004 Nov 18.

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No transcription-translation feedback in circadian rhythm of KaiC phosphorylation

Jun Tomita et al. Science. 2005.

Abstract

An autoregulatory transcription-translation feedback loop is thought to be essential in generating circadian rhythms in any model organism. In the cyanobacterium Synechococcus elongatus, the essential clock protein KaiC is proposed to form this type of transcriptional negative feedback. Nevertheless, we demonstrate here temperature-compensated, robust circadian cycling of KaiC phosphorylation even without kaiBC messenger RNA accumulation under continuous dark conditions. This rhythm persisted in the presence of a transcription or translation inhibitor. Moreover, kinetic profiles in the ratio of KaiC autophosphorylation-dephosphorylation were also temperature compensated in vitro. Thus, the cyanobacterial clock can keep time independent of de novo transcription and translation processes.

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