Oct-3/4 and Sox2 regulate Oct-3/4 gene in embryonic stem cells - PubMed (original) (raw)
. 2005 Feb 18;280(7):5307-17.
doi: 10.1074/jbc.M410015200. Epub 2004 Nov 22.
Affiliations
- PMID: 15557334
- DOI: 10.1074/jbc.M410015200
Free article
Oct-3/4 and Sox2 regulate Oct-3/4 gene in embryonic stem cells
Sayaka Okumura-Nakanishi et al. J Biol Chem. 2005.
Free article
Abstract
Oct-3/4 is a key transcriptional factor whose expression level governs the fate of primitive inner cell mass and embryonic stem (ES) cells. Previously, an upstream 3.3-kb distal enhancer (DE) fragment was identified to be responsible for the specific expression of mouse Oct-3/4 in the inner cell mass and ES cells. However, little is known about the cis-elements and trans-factors required for DE activity. In this study, we identified a novel cis-element, called Site 2B here, located approximately 30 bp downstream from Site 2A, which was previously revealed in DE by an in vivo chemical modification experiment. Using the luciferase reporter assay, we demonstrated that both Site 2A and Site 2B are necessary and sufficient for activating DE in the contexts of both the native Oct-3/4 promoter and the heterologous thymidine kinase minimal promoter. In an electrophoretic mobility shift assay we showed that Site 2B specifically binds to Oct-3/4 and Sox2 when ES-derived cell extracts were used, whereas Site 2A binds to a factor(s) present in both ES and NIH 3T3 cells. Furthermore, we showed that the physiological level of Oct-3/4 in ES cells is required for Site 2B-mediated DE activity using the inducible knock-out system of Oct-3/4 in ES cells. These results indicate that Oct-3/4 is a member of the gene family regulated by Oct-3/4 and Sox2, as reported before for the FGF-4, UTF1, Sox2, and Fbx15 genes. Thus, Oct-3/4 and Sox2 comprise a regulatory complex that controls the expression of genes important for the maintenance of the primitive state, including themselves. This autoregulatory circuit of the Sox2.Oct-3/4 complex may contribute to maintaining robustly the precise expression level of Oct-3/4 in primitive cells.
Similar articles
- Octamer and Sox elements are required for transcriptional cis regulation of Nanog gene expression.
Kuroda T, Tada M, Kubota H, Kimura H, Hatano SY, Suemori H, Nakatsuji N, Tada T. Kuroda T, et al. Mol Cell Biol. 2005 Mar;25(6):2475-85. doi: 10.1128/MCB.25.6.2475-2485.2005. Mol Cell Biol. 2005. PMID: 15743839 Free PMC article. - The gene for the embryonic stem cell coactivator UTF1 carries a regulatory element which selectively interacts with a complex composed of Oct-3/4 and Sox-2.
Nishimoto M, Fukushima A, Okuda A, Muramatsu M. Nishimoto M, et al. Mol Cell Biol. 1999 Aug;19(8):5453-65. doi: 10.1128/MCB.19.8.5453. Mol Cell Biol. 1999. PMID: 10409735 Free PMC article. - Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells.
Masui S, Nakatake Y, Toyooka Y, Shimosato D, Yagi R, Takahashi K, Okochi H, Okuda A, Matoba R, Sharov AA, Ko MS, Niwa H. Masui S, et al. Nat Cell Biol. 2007 Jun;9(6):625-35. doi: 10.1038/ncb1589. Epub 2007 May 21. Nat Cell Biol. 2007. PMID: 17515932 - Oct-4: gatekeeper in the beginnings of mammalian development.
Pesce M, Schöler HR. Pesce M, et al. Stem Cells. 2001;19(4):271-8. doi: 10.1634/stemcells.19-4-271. Stem Cells. 2001. PMID: 11463946 Review. - Interplay of Pax6 and SOX2 in lens development as a paradigm of genetic switch mechanisms for cell differentiation.
Kondoh H, Uchikawa M, Kamachi Y. Kondoh H, et al. Int J Dev Biol. 2004;48(8-9):819-27. doi: 10.1387/ijdb.041868hk. Int J Dev Biol. 2004. PMID: 15558474 Review.
Cited by
- Aberrant expression and biological significance of Sox2, an embryonic stem cell transcriptional factor, in ALK-positive anaplastic large cell lymphoma.
Gelebart P, Hegazy SA, Wang P, Bone KM, Anand M, Sharon D, Hitt M, Pearson JD, Ingham RJ, Ma Y, Lai R. Gelebart P, et al. Blood Cancer J. 2012 Aug 10;2(8):e82. doi: 10.1038/bcj.2012.27. Blood Cancer J. 2012. PMID: 22885405 Free PMC article. - Anatomy of a blastocyst: cell behaviors driving cell fate choice and morphogenesis in the early mouse embryo.
Schrode N, Xenopoulos P, Piliszek A, Frankenberg S, Plusa B, Hadjantonakis AK. Schrode N, et al. Genesis. 2013 Apr;51(4):219-33. doi: 10.1002/dvg.22368. Epub 2013 Feb 25. Genesis. 2013. PMID: 23349011 Free PMC article. Review. - Cruciform Formable Sequences within Pou5f1 Enhancer Are Indispensable for Mouse ES Cell Integrity.
Yamamoto Y, Miura O, Ohyama T. Yamamoto Y, et al. Int J Mol Sci. 2021 Mar 26;22(7):3399. doi: 10.3390/ijms22073399. Int J Mol Sci. 2021. PMID: 33810223 Free PMC article. - Cloning and Characterization of 3.1kb Promoter Region of the Oct4 Gene from the Fischer 344 Rat.
He H, McHaney M, Hong J, Weiss ML. He H, et al. Open Stem Cell J. 2009 Jan 1;1(1):30-39. doi: 10.2174/1876893800901010030. Open Stem Cell J. 2009. PMID: 22347989 Free PMC article. - Core transcriptional regulatory circuitry in human embryonic stem cells.
Boyer LA, Lee TI, Cole MF, Johnstone SE, Levine SS, Zucker JP, Guenther MG, Kumar RM, Murray HL, Jenner RG, Gifford DK, Melton DA, Jaenisch R, Young RA. Boyer LA, et al. Cell. 2005 Sep 23;122(6):947-56. doi: 10.1016/j.cell.2005.08.020. Cell. 2005. PMID: 16153702 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials