Structural evidence for variable oligomerization of the N-terminal domain of cyclase-associated protein (CAP) - PubMed (original) (raw)
. 2005 Feb 1;58(2):255-62.
doi: 10.1002/prot.20314.
Affiliations
- PMID: 15558566
- DOI: 10.1002/prot.20314
Structural evidence for variable oligomerization of the N-terminal domain of cyclase-associated protein (CAP)
Adlina Mohd Yusof et al. Proteins. 2005.
Abstract
Cyclase-associated protein (CAP) is a highly conserved and widely distributed protein that links the nutritional response signaling to cytoskeleton remodeling. In yeast, CAP is a component of the adenylyl cyclase complex and helps to activate the Ras-mediated catalytic cycle of the cyclase. While the N-terminal domain of CAP (N-CAP) provides a binding site for adenylyl cyclase, the C-terminal domain (C-CAP) possesses actin binding activity. Our attempts to crystallize full-length recombinant CAP from Dictyostelium discoideum resulted in growth of orthorhombic crystals containing only the N-terminal domain (residues 42-227) due to auto-proteolytic cleavage. The structure was solved by molecular replacement with data at 2.2 A resolution. The present crystal structure allows the characterization of a head-to-tail N-CAP dimer in the asymmetric unit and a crystallographic side-to-side dimer. Comparison with previously published structures of N-CAP reveals variable modes of dimerization of this domain, but the presence of a common interface for the side-to-side dimer.
(c) 2004 Wiley-Liss, Inc.
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