Epithelial-to-mesenchymal transition generates proliferative human islet precursor cells - PubMed (original) (raw)
. 2004 Dec 24;306(5705):2261-4.
doi: 10.1126/science.1101968. Epub 2004 Nov 25.
Affiliations
- PMID: 15564314
- DOI: 10.1126/science.1101968
Epithelial-to-mesenchymal transition generates proliferative human islet precursor cells
Marvin C Gershengorn et al. Science. 2004.
Abstract
Insulin-expressing beta cells, found in pancreatic islets, are capable of generating more beta cells even in the adult. We show that fibroblast-like cells derived from adult human islets donated postmortem proliferate readily in vitro. These mesenchymal-type cells, which exhibit no hormone expression, can then be induced to differentiate into hormone-expressing islet-like cell aggregates, which reestablishes the epithelial character typical of islet cells. Immunohistochemistry, in situ hybridization, and messenger RNA measurements in single cells and cell populations establish the transition of epithelial cells within islets to mesenchymal cells in culture and then to insulin-expressing epithelial cells.
Similar articles
- Human islet-derived precursor cells are mesenchymal stromal cells that differentiate and mature to hormone-expressing cells in vivo.
Davani B, Ikonomou L, Raaka BM, Geras-Raaka E, Morton RA, Marcus-Samuels B, Gershengorn MC. Davani B, et al. Stem Cells. 2007 Dec;25(12):3215-22. doi: 10.1634/stemcells.2007-0323. Epub 2007 Sep 27. Stem Cells. 2007. PMID: 17901402 - Generation and expansion of multipotent mesenchymal progenitor cells from cultured human pancreatic islets.
Gallo R, Gambelli F, Gava B, Sasdelli F, Tellone V, Masini M, Marchetti P, Dotta F, Sorrentino V. Gallo R, et al. Cell Death Differ. 2007 Nov;14(11):1860-71. doi: 10.1038/sj.cdd.4402199. Epub 2007 Jul 6. Cell Death Differ. 2007. PMID: 17612586 - The human insulin gene displays transcriptionally active epigenetic marks in islet-derived mesenchymal precursor cells in the absence of insulin expression.
Mutskov V, Raaka BM, Felsenfeld G, Gershengorn MC. Mutskov V, et al. Stem Cells. 2007 Dec;25(12):3223-33. doi: 10.1634/stemcells.2007-0325. Epub 2007 Sep 27. Stem Cells. 2007. PMID: 17901401 - Epithelial-mesenchymal transition in Rhesus monkey embryonic stem cell colonies: a model for processes involved in gastrulation?
Denker HW, Behr R, Heneweer C, Viebahn C, Thie M. Denker HW, et al. Cells Tissues Organs. 2007;185(1-3):48-50. doi: 10.1159/000101302. Cells Tissues Organs. 2007. PMID: 17587807 Review. - Are better islet cell precursors generated by epithelial-to-mesenchymal transition?
Gershengorn MC, Geras-Raaka E, Hardikar AA, Raaka BM. Gershengorn MC, et al. Cell Cycle. 2005 Mar;4(3):380-2. doi: 10.4161/cc.4.3.1538. Epub 2005 Mar 17. Cell Cycle. 2005. PMID: 15711124 Review.
Cited by
- Global proteomics reveals insulin abundance as a marker of human islet homeostasis alterations.
Mathisen AF, Abadpour S, Legøy TA, Paulo JA, Ghila L, Scholz H, Chera S. Mathisen AF, et al. Acta Physiol (Oxf). 2023 Oct;239(2):e14037. doi: 10.1111/apha.14037. Epub 2023 Aug 24. Acta Physiol (Oxf). 2023. PMID: 37621186 Free PMC article. - Utilization of commercial collagens for preparing well-differentiated human beta cells for confocal microscopy.
Brennecke BR, Yang U, Liu S, Ilerisoy FS, Ilerisoy BN, Joglekar A, Kim LB, Peachee SJ, Richtsmeier SL, Stephens SB, Sander EA, Strack S, Moninger TO, Ankrum JA, Imai Y. Brennecke BR, et al. Front Endocrinol (Lausanne). 2023 May 25;14:1187216. doi: 10.3389/fendo.2023.1187216. eCollection 2023. Front Endocrinol (Lausanne). 2023. PMID: 37305047 Free PMC article. - Deubiquitinase USP1 influences the dedifferentiation of mouse pancreatic β-cells.
Francis M, Bhaskar S, Komanduri S, Sheshadri P, Prasanna J, Kumar A. Francis M, et al. iScience. 2023 Apr 26;26(5):106771. doi: 10.1016/j.isci.2023.106771. eCollection 2023 May 19. iScience. 2023. PMID: 37250303 Free PMC article. - Cell Replacement Therapy for Type 1 Diabetes Patients: Potential Mechanisms Leading to Stem-Cell-Derived Pancreatic β-Cell Loss upon Transplant.
Shilleh AH, Russ HA. Shilleh AH, et al. Cells. 2023 Feb 22;12(5):698. doi: 10.3390/cells12050698. Cells. 2023. PMID: 36899834 Free PMC article. Review. - β cell regeneration and novel strategies for treatment of diabetes (Review).
Ji Z, Lu M, Xie H, Yuan H, Chen Q. Ji Z, et al. Biomed Rep. 2022 Jun 29;17(3):72. doi: 10.3892/br.2022.1555. eCollection 2022 Sep. Biomed Rep. 2022. PMID: 35950099 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical