Signaling complex formation of phospholipase Cbeta4 with metabotropic glutamate receptor type 1alpha and 1,4,5-trisphosphate receptor at the perisynapse and endoplasmic reticulum in the mouse brain - PubMed (original) (raw)
Comparative Study
Signaling complex formation of phospholipase Cbeta4 with metabotropic glutamate receptor type 1alpha and 1,4,5-trisphosphate receptor at the perisynapse and endoplasmic reticulum in the mouse brain
Michiko Nakamura et al. Eur J Neurosci. 2004 Dec.
Abstract
Upon activation of cell surface receptors coupled to the Gq subclass of G proteins, phospholipase C (PLC) beta hydrolyses membrane phospholipid to yield a pair of second messengers, inositol 1,4,5-trisphosphate (IP3) and 1,2-diacylglycerol. PLCbeta4 has been characterized as the isoform enriched in cerebellar Purkinje cells (PCs) and the retina and involved in motor and visual functions. Here we examined cellular and subcellular distributions of PLCbeta4 in adult mouse brains. Immunohistochemistry showed that high levels of PLCbeta4 were detected in the somatodendritic domain of neuronal populations expressing the metabotropic glutamate receptor (mGluR) type 1alpha, including olfactory periglomerular cells, neurons in the bed nucleus anterior commissure, thalamus, substantia nigra, inferior olive, and unipolar brush cells and PCs in the cerebellum. Low to moderate levels were detected in many other mGluR1alpha-positive neurons and in a few mGluR1alpha-negative neurons. In PCs, immunogold electron microscopy localized PLCbeta4 to the perisynapse, at which mGluR1alpha is concentrated, and to the smooth endoplasmic reticulum in dendrites and spines, an intracellular Ca2+ store gated by IP3 receptors. In the cerebellum, immunoblot demonstrated its concentrated distribution in the post-synaptic density and microsomal fractions, where mGluR1alpha and type 1 IP3 receptor were also greatly enriched. Furthermore, PLCbeta4 formed coimmunoprecipitable complexes with mGluR1alpha, type 1 IP3 receptor and Homer 1. These results suggest that PLCbeta4 is preferentially localized in the perisynapse and smooth endoplasmic reticulum as a component of the physically linked phosphoinositide signaling complex. This close molecular relationship might provide PLCbeta4 with a high-fidelity effector function to mediate various neuronal responses under physiological and pathophysiological conditions.
Similar articles
- Phospholipase Cbeta3 is distributed in both somatodendritic and axonal compartments and localized around perisynapse and smooth endoplasmic reticulum in mouse Purkinje cell subsets.
Nomura S, Fukaya M, Tsujioka T, Wu D, Watanabe M. Nomura S, et al. Eur J Neurosci. 2007 Feb;25(3):659-72. doi: 10.1111/j.1460-9568.2007.05334.x. Epub 2007 Feb 12. Eur J Neurosci. 2007. PMID: 17298601 - Immunohistochemical expressions of mGluR5, P2Y2 receptor, PLC-beta1, and IP3R-I and -II in Merkel cells in rat sinus hair follicles.
Tachibana T, Endoh M, Kumakami R, Nawa T. Tachibana T, et al. Histochem Cell Biol. 2003 Jul;120(1):13-21. doi: 10.1007/s00418-003-0540-5. Epub 2003 Jun 12. Histochem Cell Biol. 2003. PMID: 12802596 - Roles of phospholipase Cbeta4 in synapse elimination and plasticity in developing and mature cerebellum.
Hashimoto K, Miyata M, Watanabe M, Kano M. Hashimoto K, et al. Mol Neurobiol. 2001 Feb;23(1):69-82. doi: 10.1385/MN:23:1:69. Mol Neurobiol. 2001. PMID: 11642544 Review. - Homer/Vesl proteins and their roles in CNS neurons.
Ehrengruber MU, Kato A, Inokuchi K, Hennou S. Ehrengruber MU, et al. Mol Neurobiol. 2004 Jun;29(3):213-27. doi: 10.1385/MN:29:3:213. Mol Neurobiol. 2004. PMID: 15181235 Review.
Cited by
- Glyoxal fixation: An approach to solve immunohistochemical problem in neuroscience research.
Konno K, Yamasaki M, Miyazaki T, Watanabe M. Konno K, et al. Sci Adv. 2023 Jul 14;9(28):eadf7084. doi: 10.1126/sciadv.adf7084. Epub 2023 Jul 14. Sci Adv. 2023. PMID: 37450597 Free PMC article. - Nanoscale Phosphoinositide Distribution on Cell Membranes of Mouse Cerebellar Neurons.
Eguchi K, Le Monnier E, Shigemoto R. Eguchi K, et al. J Neurosci. 2023 Jun 7;43(23):4197-4216. doi: 10.1523/JNEUROSCI.1514-22.2023. Epub 2023 May 9. J Neurosci. 2023. PMID: 37160366 Free PMC article. - Long-term potentiation and depression regulatory microRNAs were highlighted in Bisphenol A induced learning and memory impairment by microRNA sequencing and bioinformatics analysis.
Luo M, Li L, Ding M, Niu Y, Xu X, Shi X, Shan N, Qiu Z, Piao F, Zhang C. Luo M, et al. PLoS One. 2023 Jan 19;18(1):e0279029. doi: 10.1371/journal.pone.0279029. eCollection 2023. PLoS One. 2023. PMID: 36656826 Free PMC article. - mGluR5 is transiently confined in perisynaptic nanodomains to shape synaptic function.
Scheefhals N, Westra M, MacGillavry HD. Scheefhals N, et al. Nat Commun. 2023 Jan 16;14(1):244. doi: 10.1038/s41467-022-35680-w. Nat Commun. 2023. PMID: 36646691 Free PMC article. - Opposite Regulation of Homer Signal at the NMJ Postsynaptic Micro Domain between Slow- and Fast-Twitch Muscles in an Experimentally Induced Autoimmune Myasthenia Gravis (EAMG) Mouse Model.
Schubert M, Pelz A, Trautmann G, Block K, Furlan S, Gutsmann M, Kohler S, Volpe P, Blottner D, Meisel A, Salanova M. Schubert M, et al. Int J Mol Sci. 2022 Nov 30;23(23):15052. doi: 10.3390/ijms232315052. Int J Mol Sci. 2022. PMID: 36499379 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous