Plant transformation: a simple particle bombardment device based on flowing helium - PubMed (original) (raw)

Plant transformation: a simple particle bombardment device based on flowing helium

Y Takeuchi et al. Plant Mol Biol. 1992 Feb.

Abstract

We observed that flowing helium at moderate pressures accelerated DNA-coated microprojectiles to velocities suitable for penetration of cells in intact plant tissues. The flowing helium principle permitted the construction of a simple and inexpensive transformation device that was easier to use than those previously described. This device provided efficient transformation of cells in soybean seedlings and other plants.

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