Germin-like protein gene family of a moss, Physcomitrella patens, phylogenetically falls into two characteristic new clades - PubMed (original) (raw)

Germin-like protein gene family of a moss, Physcomitrella patens, phylogenetically falls into two characteristic new clades

Masaru Nakata et al. Plant Mol Biol. 2004 Oct.

Abstract

We identified 77 EST clones encoding germin-like proteins (GLPs) from a moss, Physcomitrella patens in a database search. These Physcomitrella GLPs ( PpGLP s) were separated into seven groups based on DNA sequence homology. Phylogenetic analysis showed that these groups were divided into two novel clades clearly distinguishable from higher plant germins and GLPs, named bryophyte subfamilies 1 and 2. PpGLPs belonging to bryophyte subfamilies 1 lacked two cysteines at the conserved positions observed in higher plant germins or GLPs. PpGLPs belonging to bryophyte subfamily 2 contained two cysteines as observed in higher plant germins and GLPs. In bryophyte subfamily 1, 12 amino acids, in which one of two cysteines is included, were deleted between boxes A and B. Further, we determined the genomic structure of all of seven PpGLP genes. The sequences of PpGLP s of bryophyte subfamily 1 contained one or two introns, whereas those of bryophyte subfamily 2 contained no introns. Other GLPs from bryophytes, a liverwort GLP from Marchantia polymorpha , and two moss GLPs from Barbula unguiculata and Ceratodon purpureus also fell into bryophyte subfamily 1 and bryophyte subfamily 2, respectively. No higher plant germins and GLPs were grouped into the bryophyte subfamilies 1 and 2 by our analysis. Moreover, we revealed that PpGLP6 had manganese-containing extracellular superoxide dismutase activity. These results indicated that bryophyte possess characteristic GLPs, which phylogenetically are clearly distinguishable from higher plant GLPs.

PubMed Disclaimer

Similar articles

• Arabidopsis thaliana contains a large family of germin-like proteins: characterization of cDNA and genomic sequences encoding 12 unique family members.
  Carter C, Graham RA, Thornburg RW. Carter C, et al. Plant Mol Biol. 1998 Dec;38(6):929-43. doi: 10.1023/a:1006038117130. Plant Mol Biol. 1998. PMID: 9869400
• Isolation of a germin-like protein with manganese superoxide dismutase activity from cells of a moss, Barbula unguiculata.
  Yamahara T, Shiono T, Suzuki T, Tanaka K, Takio S, Sato K, Yamazaki S, Satoh T. Yamahara T, et al. J Biol Chem. 1999 Nov 19;274(47):33274-8. doi: 10.1074/jbc.274.47.33274. J Biol Chem. 1999. PMID: 10559202
• Phylogeny, function, and evolution of the cupins, a structurally conserved, functionally diverse superfamily of proteins.
  Khuri S, Bakker FT, Dunwell JM. Khuri S, et al. Mol Biol Evol. 2001 Apr;18(4):593-605. doi: 10.1093/oxfordjournals.molbev.a003840. Mol Biol Evol. 2001. PMID: 11264412
• Germins and germin like proteins: an overview.
  Patnaik D, Khurana P. Patnaik D, et al. Indian J Exp Biol. 2001 Mar;39(3):191-200. Indian J Exp Biol. 2001. PMID: 11495276 Review.
• Germin-like proteins (GLPs) in cereal genomes: gene clustering and dynamic roles in plant defence.
  Breen J, Bellgard M. Breen J, et al. Funct Integr Genomics. 2010 Nov;10(4):463-76. doi: 10.1007/s10142-010-0184-1. Epub 2010 Aug 4. Funct Integr Genomics. 2010. PMID: 20683632 Review.

Cited by

• Genome-wide identification of germin-like proteins in peanut (Arachis hypogea L.) and expression analysis under different abiotic stresses.
  Yang Q, Sharif Y, Zhuang Y, Chen H, Zhang C, Fu H, Wang S, Cai T, Chen K, Raza A, Wang L, Zhuang W. Yang Q, et al. Front Plant Sci. 2023 Jan 23;13:1044144. doi: 10.3389/fpls.2022.1044144. eCollection 2022. Front Plant Sci. 2023. PMID: 36756235 Free PMC article.
• Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis.
  Li L, Xu X, Chen C, Shen Z. Li L, et al. Int J Mol Sci. 2016 Sep 23;17(10):1622. doi: 10.3390/ijms17101622. Int J Mol Sci. 2016. PMID: 27669230 Free PMC article.
• A germin-like protein gene (CchGLP) of Capsicum chinense Jacq. is induced during incompatible interactions and displays Mn-superoxide dismutase activity.
  León-Galván F, de Jesús Joaquín-Ramos A, Torres-Pacheco I, Barba de la Rosa AP, Guevara-Olvera L, González-Chavira MM, Ocampo-Velazquez RV, Rico-García E, Guevara-González RG. León-Galván F, et al. Int J Mol Sci. 2011;12(11):7301-13. doi: 10.3390/ijms12117301. Epub 2011 Oct 25. Int J Mol Sci. 2011. PMID: 22174599 Free PMC article.
• Identification and analysis of the germin-like gene family in soybean.
  Lu M, Han YP, Gao JG, Wang XJ, Li WB. Lu M, et al. BMC Genomics. 2010 Nov 8;11:620. doi: 10.1186/1471-2164-11-620. BMC Genomics. 2010. PMID: 21059215 Free PMC article.
• Sunagoke Moss (Racomitrium japonicum) Used for Greening Roofs Is Severely Damaged by Sclerotium delphinii and Protected by a Putative Bacillus amyloliquefaciens Isolate.
  Tamura M, Tanabe M, Valkonen JPT, Akita M. Tamura M, et al. Front Microbiol. 2019 Feb 28;10:372. doi: 10.3389/fmicb.2019.00372. eCollection 2019. Front Microbiol. 2019. PMID: 30873147 Free PMC article.

References

1. 1. Plant J. 1999 Dec;20(5):541-52 - PubMed
2. 1. J Biol Chem. 1980 Jun 25;255(12):5965-70 - PubMed
3. 1. Nature. 1998 Aug 13;394(6694):671-4 - PubMed
4. 1. Gene. 1987;59(2-3):265-77 - PubMed
5. 1. Phytochemistry. 2004 Jan;65(1):7-17 - PubMed

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Ovid Technologies, Inc.

• Research Materials

  • NCI CPTC Antibody Characterization Program