Transcriptional profiling reveals complex regulation of the monocyte IL-1 beta system by IL-13 - PubMed (original) (raw)

Transcriptional profiling reveals complex regulation of the monocyte IL-1 beta system by IL-13

Chris J Scotton et al. J Immunol. 2005.

Abstract

IL-4 and IL-13 are prototypic Th2 cytokines that generate an "alternatively activated" phenotype in macrophages. We used high-density oligonucleotide microarrays to investigate the transcriptional profile induced in human monocytes by IL-13. After 8-h stimulation with IL-13, 142 genes were regulated (85 increased and 57 decreased). The majority of these genes were related to the inflammatory response and innate immunity; a group of genes related to lipid metabolism was also identified, with clear implications for atherosclerosis. In addition to characteristic markers of alternatively activated macrophages, a number of novel IL-13-regulated genes were seen. These included various pattern recognition receptors, such as CD1b/c/e, TLR1, and C-type lectin superfamily member 6. Several components of the IL-1 system were regulated. IL-1RI, IL-1RII, and IL-1Ra were all up-regulated, whereas the IL-1beta-converting enzyme, caspase 1, and IRAK-M were down-regulated. LPS-inducible caspase 1 enzyme activity was also reduced in IL-13-stimulated monocytes, with a consequent decrease in pro-IL-1beta processing. These data reveal that IL-13 has a potent effect on the transcriptional profile in monocytes. The IL-13-induced modulation of genes related to IL-1 clearly highlights the tightly controlled and complex levels of regulation of the production and response to this potent proinflammatory cytokine.

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Comment in

Arginase-1 and Ym1 are markers for murine, but not human, alternatively activated myeloid cells.
Raes G, Van den Bergh R, De Baetselier P, Ghassabeh GH, Scotton C, Locati M, Mantovani A, Sozzani S. Raes G, et al. J Immunol. 2005 Jun 1;174(11):6561; author reply 6561-2. doi: 10.4049/jimmunol.174.11.6561. J Immunol. 2005. PMID: 15905489 No abstract available.

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Transcriptional profiling reveals complex regulation of the monocyte IL-1 beta system by IL-13 - PubMed (original) (raw)